English
O'rta hujayra madaniyati jarayonida muhim oziq moddalardir. Hujayra madaniyati uchun hujayra fabrikasi dan foydalanilganda, u odatda o'rta almashinuv operatsiyasini o'z ichiga oladi, bu o'sish jarayonida hujayralar tomonidan ishlab chiqarilgan metabolik chiqindilarni olib tashlashi va hujayralar o'sishi va ko'payishini yaxshilash uchun yangi muhitni to'ldirishi mumkin.
Hujayra zavodida muhitni almashtirish uchun avvalo kerakli jihozlarni tayyorlang: spirtli ichimliklarni sug'orish uchun 75% tibbiy sterilizatsiya spirti, PBS buferi, sarum o'z ichiga olgan vosita, suyuqlik o'tkazgich qopqog'i, uzatish trubkasi, chiqindi idishi va boshqalar. Keyin barcha jihozlarni o'ta toza skameykaga qo'ying va sterilizatsiya qilish uchun UV nurlanishini yoqing. Agar o'stirilgan hujayralar haroratga sezgir bo'lsa, siz zardobni o'z ichiga olgan o'rta shishani 37 ° C dagi suv hammomiga qo'yishingiz mumkin, shunda muhit harorati 37 ° C bo'ladi, so'ngra ultrabinafsha nurlanish uchun ultra toza skameykaga o'tkazing. sterilizatsiya va UV nurlanishi ham hujayra kulturalari orasida taxminan yarim soat davomida talab qilinadi.
Ultrabinafsha nurlanish bilan sterilizatsiya qilingandan so'ng, operator sterilizatsiya kiyimi, sterilizatsiya qo'lqoplari va niqoblarini kiydi, hujayra zavodini olib chiqdi, yuzasiga 75% spirt purkadi. dezinfeksiya uchun alkogolli sug'orish idishi bilan, va uni o'ta toza skameykaga o'tkazing, qopqog'ini ochib, Suyuqlik o'tkazgich qopqog'ini va uzatish trubkasini eksport madaniyat muhitiga ulang. Yangi sarum o'z ichiga olgan vositani qo'shing, uni hujayralarning yopishgan yuzasidan emas, balki yon devoridan qo'shmaslik uchun ehtiyot bo'ling va hujayralarni yuvmaslik uchun yumshoq harakat qiling. Muhitni qo'shgandan so'ng, qopqog'ini yoping, sarf materiallari yuzasini belgilang va vositani o'zgartirish vaqtini, hujayra turini, operatorni va boshqa ma'lumotlarni ko'rsating. Uni inkubatorga qo'yishdan oldin sirtni yana spirt bilan püskürtün, operatsiya stolini tozalang, ultra toza stolni spirt bilan artib, chiqindi suyuqlik va axlatni tozalang.
Hujayralar atrof-muhitga sezgir. Hujayra zavodida muhitni o'zgartirganda, muhitni o'zgartirish jarayonida turli bakteriyalarning infektsiyasi tufayli hujayra o'sishiga ta'sir qilmaslik uchun aseptik ishlashga e'tibor berish kerak.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.