English
Hujayra madaniyati texnologiyasi biofarmatsevtika, monoklonal antikorlar va hujayra terapiyasi sohalarida keng qo'llaniladigan texnologiyadir. Hujayra fabrikalari asosan katta hajmdagi hujayra madaniyati uchun ishlatiladi. Hujayralarning o'sishiga ko'plab omillar ta'sir qiladi, bulardan harorat juda muhim jihatdir.
Oddiy sharoitda sutemizuvchilar va parrandalar hujayralarini in vitro ekish uchun mos harorat 37-38 °C ni tashkil qiladi. Juda yuqori yoki juda past harorat hujayralar o'sishiga ta'sir qiladi. Hujayralarning past haroratga bardosh berish qobiliyati issiqlik qarshiligiga qaraganda kuchliroqdir. Past haroratda hujayralarning metabolik faolligi va yadro bo'linishi kamayadi. Harorat 0 ° C dan past bo'lmaganda, hujayra metabolizmiga ta'sir qilsa-da, u zararli ta'sir ko'rsatmaydi; hujayralar 25 dan 35 ° C gacha bo'lgan haroratda joylashtirilsa, hujayralar hali ham omon qolishi va o'sishi mumkin, ammo tezlik sekinlashadi; 37 ℃ ga qaytish madaniyat hujayralari o'sishda davom etishi mumkin.
Yuqori harorat hujayra madaniyati uchun yaxshi emas. Hujayralar 39-40°S da 1 soat davomida yetishtiriladi, ular ma'lum darajada zararlanishi mumkin, lekin ular baribir tiklanishi mumkin, lekin ular bir necha soat davomida haroratning 2°C ga ko'tarilishiga chidamaydilar, ya'ni ekilgan bo'lsa. 41-42 ° S haroratda 1 soat davomida hujayra shikastlanishi kuchli. Hujayralarning aksariyati harorat 43 ° C dan yuqori bo'lganida nobud bo'lgan. Yuqori harorat asosan fermentlarning inaktivatsiyasiga, lipidlarning yo'q qilinishiga, yadro bo'linishining buzilishiga, koagulaza ishlab chiqarilishiga, hujayralarning koagulyatsiyasiga va oqsillarning denaturatsiyasiga olib keladi. Shuning uchun hujayra zavodi tegishli haroratni saqlab turishi va hujayralarni o'stirishda yuqori haroratdan qochish kerak.
Xulosa qilib aytganda, hujayra zavodida hujayra o'sishiga ko'p omillar ta'sir qiladi va harorat ulardan faqat bittasi. Boshqalarga osmotik bosim, steril muhit, gaz va pH kiradi.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.