English
Turli hujayralarni etishtirishda hujayra madaniyati flasks tez-tez ishlatiladigan hujayra madaniyati sarf materiallari turidir. Ular asosan yopishgan hujayralar madaniyati uchun ishlatiladi va texnik xususiyatlar 25 ml dan 225 ml gacha. Hamster buyrak hujayra madaniyati muhiti ushbu sarflanadigan materialdan foydalanadi va uni etishtirishning o'ziga xos bosqichlari quyidagilardan iborat:
1. Hujayra madaniyatiga tayyorgarlik ishlari:
1) MEM muhitini tayyorlash; yuqori sifatli homila sigir zardobi, 10%; qo'sh antikor 1%.
2) Madaniyat sharoitlari: Gaz fazasi: havo, 95%; karbonat angidrid, 5%. Harorat: 37 daraja Selsiy, inkubator namligi 70%-80%.
3) Muzlatish eritmasi: 90% sarum, 10% DMSO, foydalanishga tayyor.
2. Hujayrani qayta ishlash:
1) Kriopservalangan hujayralarni qayta tiklash: 1 ml hujayra suspenziyasi bo'lgan kriyokonservalangan trubkani 37°C suv hammomida tez silkitib, eritib, 4-6 ml to'liq muhitni o'z ichiga olgan sentrifuga trubasiga qo'shing va aralashtiring. yaxshi. 3-5 daqiqa davomida 1000 RPM da santrifüj qiling, supernatantni tashlang va hujayralarni to'liq muhitda qayta suspenziya qiling. Keyin hujayra suspenziyasi 6-8 ml to'liq muhitni o'z ichiga olgan hujayra madaniyati kolbasiga qo'shildi va bir kechada 37 ° C da madaniyatlandi. Hujayra o'sishi va hujayra zichligi ertasi kuni mikroskop ostida kuzatildi.
2) Hujayra o'tishi: Hujayra zichligi 80%-90% ga yetsa, uni subkulturalash mumkin.
3) Hujayralarning kriokonservatsiyasi: Qabul qilingandan so'ng. hujayra urug'lari partiyasini keyingi tajribalar uchun kulturaning dastlabki 3 o'tishida muzlatish tavsiya etiladi.
Yuqoridagilar hujayra madaniyati kolbalarida hamster buyrak hujayralarini etishtirishning o'ziga xos bosqichlari. Bundan tashqari, hujayralar kriyokonservalanganda, hazm qilingan hujayralarni hujayra o'tish jarayoniga ko'ra sentrifuga trubkasiga to'plang va hujayralarning kriosaqlanish zichligini aniqlash uchun ularni hisoblash uchun gemositometrdan foydalaning. Umumiy hujayralarning tavsiya etilgan muzlatish zichligi 1 × 106 ~ 1 × 107 jonli hujayralar / ml ni tashkil qiladi.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.