In vitro ekilgan hujayralarning aksariyati yopishgan hujayralardir. Bunday hujayralarning o'sishi biriktirilishi mumkin bo'lgan qo'llab-quvvatlash yuzasiga ega bo'lishi kerak. Hujayralar o'z-o'zidan ajralib chiqadigan yoki madaniy muhitda ta'minlangan biriktiruvchi omillarga tayanib, sirtda o'sishi va ko'payishi mumkin. Shunday qilib, yopishgan hujayralarning hujayra madaniyati sarf materiallariga qanday talablar qo'yiladi?
Ankrajga bog'liq bo'lgan hujayralar ekilganda madaniyat (shisha) idishining devoriga biriktirilishi kerak. Hujayralar biriktirilgach, ular tez tarqaladi, keyin mitoz boshlanadi va tezda logarifmik o'sish bosqichiga o'tadi. Bir necha kundan so'ng hujayralar madaniy idishning yuzasini qoplaydi va zich hujayrali mono qatlam hosil qiladi. Yopishqoq hujayralar uchun ishlatiladigan hujayra madaniyati sarf materiallari hujayra zavodlari, hujayra madaniyati plitalari, hujayra yigiruvchi kolbalar va boshqalarni o'z ichiga oladi. Hozirgi vaqtda bozordagi hujayra sarf materiallarining aksariyati asosan PS, PC va PE kabi polimer materiallardir. Ushbu turdagi materiallar shishaning hidrofilik xususiyatlariga ega emas va yopishtiruvchi hujayralarni etishtirish uchun ishlatishdan oldin sirtni maxsus ishlov berish kerak. Maxsus ishlov berilgan hujayra madaniyati sarf materiallari yuzasiga kiritilgan gidrofil guruhga ega bo'lib, bu hujayralarga sarf materiallari yuzasiga yaxshiroq yopishish imkonini beradi, bu hujayra o'sishi va ko'payishi uchun qulaydir.
Hujayra o'sishi uglerod kabi o'ziga xos muhitni talab qiladi. dioksid, pH, sterillik, osmotik bosim va h.k., shuning uchun hujayra madaniyatining rivojlanishiga ta'sir qiladigan hujayra ifloslanishining oldini olish uchun foydalanishdan oldin hujayra madaniyati sarf materiallari sterilizatsiya qilinishi va DNazsiz va RNazsiz sharoitlarga yetishi kerak.
Surface TC davolash va sterilizatsiya yopishgan hujayralar uchun hujayra madaniyati sarf materiallari uchun ikkita asosiy talab bo'lib, ular ham hujayra madaniyati ishining silliq rivojlanishi uchun zarur shartlardir. Shuning uchun, iste'mol qilinadigan mahsulotlarni tanlashda siz ko'proq ehtiyot bo'lishingiz kerak.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.