English
- bu hayotni tadqiq qilish va amaliyotda qo'llaniladigan hujayra sarf materiallarining bir turi. U asosan yopishgan hujayralarni etishtirish uchun ishlatiladi. Foydalanish jarayonida to'g'ri ishlash usuli eksperimentning silliq rivojlanishini ta'minlashning zaruriy shartidir. Ushbu sarflanadigan materialdan foydalanganda quyidagilarga e'tibor bering: soat necha:1. Qopqoq haqida: Gaz almashinuvini ta'minlash uchun bir martalik hujayra madaniyati kolbasining qopqog'ini juda qattiq siqmang. HEPES 7,2-7,4 pH oralig'ida yaxshi buferlash qobiliyatiga ega bo'lgan ion bo'lmagan buferdir, lekin u juda qimmat va yuqori konsentratsiyalarda ba'zi hujayralar uchun toksik bo'lishi mumkin. HEPES buferi HEPES qo'shilishi natijasida yuzaga keladigan osmotik bosimning oshishini bartaraf etish uchun past darajadagi natriy karbonat (0,34 g / L) bilan ishlatilishi mumkin. Ushbu madaniyat sharoitida, kultura eritmasidagi oz miqdordagi karbonatning havoga tarqalishini oldini olish uchun bir martalik hujayra kulturasining qopqog'ini mahkamlash kerak. Ko'pchilik kultura suyuqliklarida pH indikatori sifatida fenol qizil, kislotali kultura suyuqliklari to'q sariq-sariq, ishqoriy kultura suyuqliklari esa to'q qizil rangga ega.hujayra kulturasi
2. "O'sish bo'shlig'ini" kamaytiring: Ko'pchilik foydalanuvchilar oldindan qoplangan bir martalik hujayra madaniyati kolbalaridan emas, balki oddiy bir martalik hujayra madaniyati kolbalaridan foydalanadilar. Sirt muhiti zardobsiz madaniyat muhitida mezenximal ildiz hujayra pastasi uchun qulay emas. devor. Madaniy muhitdagi yopishqoqlikni rag'batlantiruvchi moddalarni bir martalik hujayra kulturasining pastki qismi bilan yaxshiroq birlashtirish uchun uni 37 ° C haroratda 5-10 daqiqa davomida joylashtiring, bu mezenximal ildiz hujayralarining yopishish qobiliyatini oshiradi va "o'sish teshiklari" ehtimolini kamaytiradi. ".
3. Hujayra hazmi: shishani tik qo'ying va madaniy muhitni so'rib oling. Madaniyat muhitida ko'proq eksfoliatsiyalangan hujayralar bo'lsa, bu hujayralar endi tushishi oson ekanligini anglatadi. Shunchaki 1 ml PBS+EDTA (0,02%) qo'shing va hujayralar to'liq tushib ketguncha madaniyat shishasini oldinga va orqaga biroz silkiting, 10 ml MEM qo'shing, yaxshilab aralashtiring, pipetka qilmang, 2 shishaga bo'ling, agar hujayralar o'smasdan tushib qolsa, faqat 5ml MEM qo'shing, alohida qadoqlash yo'q. Agar shishadagi hujayralar mahkam yopishib qolsa, o'rta supernatantda hujayra qoldiqlari bo'lmasa va hujayralar 80% dan oshib ketgan bo'lsa, muhitni aspiratsiya qiling, 5 ml PBS + EDTA (0,02%) qo'shing, tezda uni biroz chayqatib, kultura qiling. tik holatda shishani aspiratsiya qiling, 1 ml 0,05% pankreatin qo'shing, 37 ° C da 3 daqiqadan ko'p bo'lmagan vaqt davomida hazm qiling va hujayralar butunlay hazm qilinadi va tozalanadi. Chiqib oling va muloyimlik bilan pipetlash va aralashtirish uchun 10 ml madaniyat muhitini qo'shing va 2 shishaga bo'ling.
Hujayralar o'sish muhitiga juda sezgir. Ko'proq ishlatiladigan sarf materiallari sifatida
hujayra o'sishiga ta'sir qiladigan noto'g'ri operatsiyalarni oldini olish uchun ehtiyotkorlik bilan va belgilangan tartiblarga qat'iy rioya qilish kerak.cell culture flasks hujayra madaniyati kolbalari
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.