English
Hujayra o'tishi kulturani kichik bo'laklarga bo'lish, boshqa madaniyat idishida qayta ekish va keyin ekish jarayonini anglatadi. Hujayra kulturalari o'tish jarayonida keng tarqalgan iste'mol qilinadi, shuning uchun hujayra o'tishining o'ziga xos bosqichlari qanday?
1. Inkubatordan hujayra madaniyati kolbalarini chiqarib oling, shisha yuzasiga 75% li spirtni spirt purkagich bilan seping va uni o'ta toza stolga o'tkazing.
2. Qopqoqni oching, eski madaniyat muhitini muloyimlik bilan aspiratsiya qiling, Paster pipetkasi bilan 1-2 marta yuvish uchun tegishli miqdorda PBS buferini qo'shing va PBS buferini tashlang.
3. 1-2 ml pankreatin qo'shish uchun pipetkadan foydalaning va pankreatin hujayralarga to'liq tegishi uchun "xoch" ni silkiting.
4. Tripsin solingan hujayra madaniyati kolbasini teskari mikroskop bosqichiga o'tkazing va mikroskop ostida hujayra hazmini kuzating. Hujayralar yumaloq bo'lib, ko'p miqdorda tushganda, ovqat hazm qilishni to'xtatishga tayyorlaning. Shishaning yuzasiga 75% spirt seping va uni super Net Taiwanga o'tkazing.
5. Ovqat hazm qilishni tugatish uchun teng miqdorda sarum o'z ichiga olgan vositani qo'shish uchun pipetkadan (yoki Paster pipetkasidan) foydalaning. Hujayralar to'liq tushib ketishi uchun pipetka bilan yaxshilab torting.
6. Shishadagi aralash suyuqlikni santrifuga trubasiga o'tkazing, uni muvozanatlang va taxminan 5 daqiqa davomida santrifüj qiling.
7. Santrifüjdan so'ng, santrifüj trubkasi yuzasiga spirtli purkagich bilan püskürtün, uni o'ta toza stolga o'tkazing, qopqog'ini oching va supernatantni chiqindi idishga quying.
8. Zardob o'z ichiga olgan vositaning tegishli hajmini qo'shing va hujayra suspenziyasini tayyorlash uchun pipetka yoki Paster pipetkasi bilan muloyimlik bilan pipetka qiling.
9. Hujayra suspenziyasini 2 (yoki undan ko'p) toza steril kulturaga bo'ling, tegishli miqdorda madaniy muhit qo'shing va qopqog'ini yoping.
10. Alikotlangan kulturani teskari mikroskop sahnasiga qo'ying va hujayra holatini kuzating. Hujayralar soni kafolatlangan bo'lishi kerak. Juda kichik raqam o'sishga ta'sir qiladi.
11. O'tish vaqti, hujayra turi, operator va boshqa ma'lumotlarni ko'rsatish uchun shishani belgilang. Uni inkubatorga qo'yishdan oldin shisha korpusining yuzasini yana spirt bilan püskürtün, operatsiya stolini tozalang, ultra toza stol yuzasini 75% spirt bilan artib, chiqindi suyuqlik va axlatni tozalang.
The yuqorida hujayra o'tish uchun hujayra kulturalari dan foydalanishning o'ziga xos jarayoni. Esda tutingki, siz operatsiya vaqtida steril kiyim, steril qo'lqop va niqob kiyishingiz va hujayralar ifloslanishini oldini olish uchun jarayon davomida aseptik operatsiyalarga e'tibor berishingiz kerak.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.