English
Subkultura madaniyatni kichik qismlarga bo'lish, ularni boshqa madaniyat idishiga (shisha) qayta emlash va keyin kultivatsiya qilish jarayonini anglatadi. Hujayra kulturalari - hujayralarni etishtirishda keng tarqalgan ishlatiladigan sarf materiallari. Demak, kolbadagi hujayralarni subkulturalash qanday amalga oshiriladi?
Umuman olganda, hujayra kulturalaridagi hujayralar zich bir qatlamga aylanadi va asosan toʻyingan boʻladi. Hujayralarning ko'payishda davom etishi va hujayralar sonini kengaytirishi uchun o'tish operatsiyalarini bajarish kerak. To'xtatilgan hujayralarni to'g'ridan-to'g'ri ajratish mumkin, yopishgan hujayralarni ajratishdan oldin hazm qilish kerak. Odatda, proteolitik fermentlar (masalan, tripsin) yopishgan hujayralarni bitta hujayralarga hazm qilish uchun ishlatiladi. Ovqat hazm qilishni yaxshilash uchun etilendiamintetraasetik kislota (EDTA) ham qo'shilishi mumkin. Hujayralar yumaloq bo'lguncha va shisha devoridan ajralib ketguncha hujayralarni kuzating. Bu jarayon ko'pincha 5-15 daqiqa davom etadi. Hujayra to'planishining oldini olish uchun hujayralarni hazm qilish jarayonida hujayra madaniyati kolbalariga tegmang.
Ayniqsa, hazm qilish qiyin bo'lgan hujayralar uchun siz tripsin EDTA hazm qilish eritmasini qo'shishingiz va hujayralarni 37 ° C haroratgacha joylashtirishingiz mumkin. hazm qilishni osonlashtiradi. Hujayralar shisha devoridan ajratilgandan so'ng, ovqat hazm qilishni to'xtatish uchun darhol sarumni o'z ichiga olgan to'liq vositani qo'shing. Sarumdagi ba'zi oqsillar oshqozon osti bezini inhibe qilishi mumkin. Ferment faolligi. Umuman olganda, santrifüjlash shart emas. Agar hujayralar zardobsiz yoki past zardobli muhitga muhtoj bo'lsa, 5 daqiqa davomida 125000 g da santrifüj qiling, so'ngra to'xtatilgan muhitni tashlang, tegishli yangi muhit qo'shing va subkulturadan oldin hujayralarni muloyimlik bilan aralashtiring.
Hujayra o'tish nisbati odatda 1:2 ni tashkil qiladi. -1:20, hujayra turiga qarab. O'tish jarayonida tripsin ba'zi hujayralarning hujayra membranasiga zarar etkazadi. Hujayralarni muloyimlik bilan qirib tashlash uchun hujayra qirg'ichidan foydalanishingiz mumkin, mos miqdorda vosita qo'shing, hujayralarni aralashtirish uchun muloyimlik bilan pipetka bilan o'tkazing va ularni yangi madaniyat kolbasiga o'tkazing.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.