Hujayra madaniyati energiya sarflaydigan maxsus vazifadir. Bu nafaqat mos harorat, osmotik bosim, gaz, pH qiymati, madaniyat muhiti va boshqa sharoitlarni, balki hujayra o'sishini muntazam ravishda kuzatishni ham talab qiladi. Shunday qilib, hujayra fabrikasi kabi ko'p qatlamli tuzilishga ega bo'lgan sarf materiallari uchun hujayralarning o'sish holatini qanday kuzatasiz?
Hujayra zavodi yetishtirilganda hujayra o'sishi holatini kuzatish mumkin teskari mikroskop yoki hujayra mikroskopik kuzatuv monitori. Farqi shundaki, teskari mikroskop faqat pastki 1-2 qavatdagi hujayra o'sishini kuzatishi mumkin, hujayra mikroskopik kuzatuv detektori esa yuqori aniqlikdagi sovuq nurli LED chiroqlar yordamida ko'proq hujayralar o'sishini kuzatishingiz mumkin.
Yaxshi o'sish holatidagi hujayralarni oddiy mikroskop ostida kuzatuv ostida ko'rish mumkin. Hujayralarning shaffofligi katta va kontur aniq emas. Faqatgina kontrastli fazali mikroskop yordamida hujayralarning nozik tuzilishini aniq ko'rish mumkin. Hujayralarda zarrachalar kam, vakuolalar, hujayra membranalari tiniq, kultura muhiti tiniq va shaffof, osilgan hujayralar va qoldiqlar ko‘rinmaydi; hujayra funktsiyasi yomonlashganda kontur kuchayadi, sitoplazmada ko'pincha vakuolalar, lipid tomchilari va boshqa zarrachalar paydo bo'ladi. Ularning orasidagi bo'shliqning ortishi bilan hujayra morfologiyasi tartibsiz bo'lib qolishi yoki hatto o'zining asl xususiyatlarini yo'qotishi mumkin.
Hujayralarning o'sish holatini kuzatish hujayra zavodi kulturasi uchun zaruriy qadamdir. Tajribalarda faqat yaxshi o'sish holatidagi hujayralardan foydalanish mumkin. Odatda, hujayra inokulyatsiyasi yoki o'tishidan so'ng, hujayra morfologiyasini, o'sishini, madaniyat muhitining pH darajasini va ifloslanishni kuzatib boring yoki har kuni yoki kamida 1-2 kun davomida kuzating va o'rta almashtirish yoki o'tishni davolash uchun hujayralarning dinamik o'zgarishlarini kuzatib boring. . Agar g'ayritabiiy holatlar aniqlansa, darhol choralar ko'ring.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.