English
- hujayra madaniyati sarf materiallarining bir qatlamli yoki ko'p qatlamli tuzilishi. Hujayra madaniyati jarayonida keyingi bosqichga o'tishdan oldin hujayralarning o'sish holatini vaqtida tushunishimiz kerak. Hayotiy hujayralar sonini tripan ko'k bo'yash orqali aniqlash mumkin. Muayyan ish usuli quyidagicha:1. Birikkan hujayralar uchun muhitni aspiratsiya qiling, hazm qilish eritmasini qo'shing va tegishli vaqt uchun inkubatorda hazm qiling. Hujayralar yumaloq bo'lgandan so'ng, hazm qilish hazm qilish hajmiga teng yoki undan kattaroq muhit qo'shilishi bilan tugatildi. Santrifüjdan keyin hujayra suspenziyasi madaniy muhit bilan tayyorlanadi; hujayra fabrikasi
2. 0,5 ml tripan ko'k eritmasi, 0,3 ml PBS va 0,2 ml hujayra suspenziyasini oling va yaxshilab aralashtiring. Keyin aralash 1-2 daqiqaga qoldiriladi. Aralashmaning oz miqdorini tortib, gemositometrga AOK qilish uchun kapillyar pipetka yoki 10 ul kichik pipetka uchidan foydalaning;
3. Kamida 1000 ta hujayrani hisoblang va bo'yalgan hujayralar sonini hisoblang;
4. Hisoblash plastinkasini va qoplamani o'ta toza suv va 75% etanol bilan yuving;
5. Oddiy hujayralar bo'yalmaydi, faqat hujayralar o'lganidan keyin hujayra membranasining o'tkazuvchanligi kattalashadi, tripan ko'k hujayralarga kiradi va hujayralar ko'k rangga ega bo'ladi. Aralash uzoq vaqt davomida joylashtirilmasligi kerak, aks holda oddiy tirik hujayralar ham bo'yoqni oladi, bu esa hisoblash aniqligiga ta'sir qiladi;
6. Hujayra hayotiyligi (%) = (hujayralarning umumiy soni - bo'yalgan hujayralar soni) / hujayralarning umumiy soni*100;
7. Hujayralarni hisoblash usuli kabi, tripan ko'k bo'yashda katta xatolik bor. U faqat erta apoptoz darajasini aniqlay oladi va nazorat guruhi va eksperimental guruh o'rtasidagi farqni ko'rsata olmaydi.
Hujayra zavodida hujayralarni etishtirish jarayonida har doim turli sabablarga ko'ra nobud bo'ladigan ba'zi hujayralar mavjud. Tirik hujayralar soni va hujayra hayotiyligini o'zlashtirish hujayra madaniyati tajribalarida asosiy operatsiya va tajribaning rivojlanishini ta'minlashning samarali usuli hisoblanadi.
In the process of culturing cells in a cell factory, there are always some cells that die due to various reasons. Mastering the number of living cells and cell viability is a key operation in cell culture experiments and an effective way to ensure the progress of the experiment.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.