English
Hujayra kulturalariodatda yopishgan hujayra madaniyatida ishlatiladigan hujayra madaniyati sarf materiallarining bir turi. Yopishqoq hujayralar suspenziya hujayralaridan farq qiladi va o'sishi uchun tayanch yuzasiga biriktirilishi kerak. Adderens ta'siri bevosita hujayralar o'sishiga ta'sir qiladi va hujayralarning adderens ta'sirini quyidagi usullar bilan yaxshilash mumkin:
1. Hujayralarning tegishli hazm bo'lishi: HepG-2, A549, Hela, SGC-7901 saraton hujayralari uzoqroq vaqt davomida davolanishi mumkin. Odatda, davolanish vaqti 5-6 minut. C2C12, COS-7, NIH-3T3 tushishi osonroq va 2 daqiqa etarli. , P19Cl6 va 293 hujayralar mahkam yopishmaydi, shuning uchun ularni tarqatish uchun PBSda yuvilgandan so'ng to'g'ridan-to'g'ri yangi muhit bilan almashtirilishi mumkin.
2. Iloji boricha plastik hujayra madaniyati idishlaridan foydalaning. Bunday shishalar TC bilan ishlov berilgandan so'ng, shishalarning yopishish ko'rsatkichlari sezilarli darajada yaxshilanadi va hujayralar ularga yaxshiroq biriktirilishi mumkin.
3. Yangi hujayralarni reanimatsiya qiling va birinchi haftada hujayralarning tiklanishiga yordam berish uchun 20% sarumdan foydalaning.
4. Hujayralarning devorga yaxshiroq yopishishiga yordam berish uchun tegishli hujayradan tashqari matritsa yoki adezyon reagentlaridan foydalaning.
5. Hujayra yopishqoqligi ta'siriga ta'sir qilmaslik uchun hujayralarni 24 soat ichida silkitmaslikka harakat qiling.
6. Tanadagi epiteliya hujayralari kollagendan tashkil topgan bazal membranada o'sadi, shuning uchun kollagen bilan substratda etishtirish o'sish uchun foydalidir. Odam yoki sichqon epidermal hujayra madaniyati uchun g-nurlari bilan nurlangan 3T3 hujayralari oziqlantiruvchi qatlam sifatida ishlatilishi mumkin va pH, Ca2+ miqdori va harorat kamayadi, bu epiteliya hujayralarining o'sishi uchun foydalidir.
Bir necha usullar mavjud. hujayra kulturasi ichidagi hujayralarning yopishish ta'sirini yaxshilash uchun. Bundan tashqari, hujayra to'planib o'sishiga yo'l qo'ymaslik uchun subkultura paytida bir tekis tarqalishni unutmang.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.