English
"Edge effekti" - bu 96-quduqli plitalar, dan foydalanish paytida, ayniqsa ferment bilan bog'liq immunoreaktsiyalarda (ELISA tajribalari) tez-tez uchraydigan holat. Bu holat chekka teshiklardagi hujayralarning o'sish tezligini sekinlashishiga olib keladi, bu esa sinov natijalariga bevosita ta'sir qiladi.
Bu vaziyatni quyidagi yo'llar bilan hal qilish mumkin:
1. Oldindan inkubatsiya: suv hammomidan foydalaning yoki reaktsiya eritmasini 96-quduqli plastinkaga qo'shganda, plastinka va eritmani inkubatsiya haroratiga (masalan, 37 ° C) qizdiring, bu "chekka effekti" ni osongina yo'q qilishi va yaxshilanishi mumkin. o'lchovning takrorlanishi. Bundan tashqari, ba'zi tadqiqotlar shuni ko'rsatdiki, asfaltlangan hujayralarni xona haroratida bir soat davomida joylashtirish va hujayralar joylashib, devorga yopishganidan keyin kultivatsiyani davom ettirish uchun ularni 37 ° C inkubatorga qaytarish ham chekka ta'sirini sezilarli darajada yaxshilashi mumkin.
2. Anti-uchuvchi qopqoqdan foydalaning: Ba'zi odamlar gözenekli plastinka ning uchuvchanlik ta'siridan kelib chiqadigan chekka effekt uchun uchuvchanlikka qarshi teshilgan qopqoqni ishlab chiqdilar. Teshilgan plastinka qopqog'i bug' qalqoni bo'lishi uchun teshilgan plastinkada to'yingan suv bug'i qatlamini hosil qilishi va teshilgan plastinkani nisbatan barqaror holatda yaxshiroq ushlab turish uchun teshilgan plastinka ichidagi muhitni laboratoriya muhitidan ajratib qo'yishi mumkin.
3. Periferik teshiklardan voz keching: Yuqoridagi usullarni hal qilib bo'lmaganda, ishonchliroq sinov natijalarini olish uchun, eng oddiy va oddiy usul bu periferik teshiklardan foydalanmaslikdir.
Yuqoridagilar @__ da chekka ta'sirlarni oldini olish uchun echimlardir. @. Ushbu qayta ishlash usullarini o'zlashtirish chekka effektlarning sinov natijalariga ta'sirini kamaytirishi mumkin.96-well plates96-quduqli plitalar
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.