English
Hujayra madaniyati hujayralarni emlash, etishtirish, yig'ish va boshqa jarayonlarni o'z ichiga oladi. hujayra kulturalari dagi hujayralar ma'lum darajada o'sgan va hujayralarni yig'ib olish kerak. Bu vaqtda kolba devoridagi hujayralarni tripsin bilan hazm qilish va keyin yig'ib olish kerak. Pankreatinni qo'llash paytida ovqat hazm qilish vaqtini nazorat qilish kerak.
Tripsin heterojen proteaz bo'lib, hujayra membranasi va hujayra madaniyati kolbalaridagi oqsilni parchalashi mumkin. Proteinni ma'lum bir joyda parchalash orqali oqsillar o'rtasidagi tutashuvdagi oqsil parchalanadi va hujayralar ichki sitoskeletning tarangligi tufayli sharsimon bo'lib, hujayralarni ajratib turadi. Tripsinni hazm qilish darajasi hujayra madaniyatida asosiy bosqich hisoblanadi: haddan tashqari hazm qilingan hujayra qoldiqlari ko'payadi, qora ko'pik ko'payadi, hujayralar bo'laklarga bo'linadi, bu hujayra hayotiyligiga jiddiy ta'sir qiladi va ba'zi hujayralar tashlab yuborilgan tripsin bilan birga suzadi va yo'qotadi; Ovqat hazm qilish yetarli emas, hujayralar shisha devoridan puflash qiyin, takroriy pipetlash ham hujayra hayotiyligiga putur etkazadi.
Turli to'qimalar yoki hujayralar pankreatin ta'siriga turlicha munosabatda bo'lishadi. Pankreatin dispers hujayralarining faolligi uning konsentratsiyasi, harorati va ta'sir qilish vaqti bilan ham bog'liq. Agar pH 8,0 va harorat 37 bo'lsa, pankreatin eritmasining ta'sir qilish qobiliyati nisbatan kuchli. Pankreatinni qo'llashda ortiqcha ovqat hazm qilish natijasida hujayralar shikastlanishiga yo'l qo'ymaslik uchun konsentratsiyani, haroratni va vaqtni nazorat qilish kerak. Ca2+, Mg2+, sarum va oqsil pankreatin faolligini kamaytirishi mumkinligi sababli, pankreatin eritmasi, masalan, D-Hanks eritmasini tayyorlashda Ca2+ va Mg2+ bo'lmagan BSS dan foydalanish kerak. Ovqat hazm qilishni to'xtatganda, pankreatinning hujayralarga ta'sirini to'xtatish uchun sarum o'z ichiga olgan madaniy muhit yoki tripsin inhibitori foydalaning.
Bundan tashqari, agar u yangi sotib olingan hujayra bo'lsa, ovqat hazm qilish jarayonini aniqlash uchun birinchi navbatda past konsentratsiyali tripsindan foydalanishingiz mumkin. vaqtni belgilang va hujayralar har daqiqada dumaloq yoki yo'qligini kuzatib boring va rekord qo'ying. Muxtasar qilib aytganda, hujayra madaniyati kolbalari ichidagi hujayralarni yig'ishda, hujayra hayotiyligini saqlab qolish uchun tripsin hazm qilish vaqtini nazorat qilish kerak.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.