English
Hujayralarni etishtirish jarayonida muhitni o'zgartirish juda muntazam operatsiya bo'lib, u asosan hujayra o'sishi jarayonida hosil bo'lgan turli xil metabolik chiqindilarni olib tashlash, yangi madaniyat muhitini to'ldirish va hujayra o'sishini rag'batlantirish uchun ishlatiladi. Shunday qilib, hujayra madaniyati kolbalaridagi eritmani qanday o'zgartirish mumkin?
Tayyorlanishi: 75% tibbiy dezinfeksiya spirti, PBS buferi, sarumli muhit, pasterizatsiyalangan pipetka, pipetka, chiqindi suyuqlik idishi bo'lgan alkogolli purkagich. .
Maxsus qadamlar:
1. Uskunani ultra toza stolga qo'ying va sterilizatsiya qilish uchun ultrabinafsha nurlanishni yoqing. Agar o'stirilgan hujayralar haroratga sezgir bo'lsa, madaniyat muhiti harorati 37 ℃ bo'lishi uchun zardob o'z ichiga olgan madaniy muhit shishasini 37 ℃ suv hammomiga qo'yishingiz mumkin va keyin ultrabinafsha nurlanish sterilizatsiyasi uchun ultra toza skameykaga o'ting. Bundan tashqari, hujayra madaniyati xonasi ham taxminan yarim soat davomida UV nurlanishiga muhtoj.
2. Inkubatordan hujayra madaniyati kolbalarini chiqarib oling, dezinfeksiya qilish uchun shisha yuzasiga 75% spirtni spirt purkagich bilan seping, o'ta toza stolga o'tkazing, qopqog'ini oching, eski madaniyat muhitini muloyimlik bilan so'rib oling, yangi zardob o'z ichiga olgan muhitni qo'shish uchun pipetka, Madaniyat muhitini hujayralarning yopishgan yuzasidan emas, balki yon devoridan ehtiyotkorlik bilan, hujayralarni yuvib yubormaslik uchun qo'shing.
3. Madaniyat muhitini qo'shgandan so'ng, qopqog'ini yoping va muhitni, hujayra turini, operatorni va boshqa ma'lumotlarni o'zgartirish vaqtini ko'rsatish uchun shishani belgilang.
4. Uni inkubatorga qo'yishdan oldin shisha yuzasiga yana spirt seping, so'ngra operatsiya stolini tartibga solishni, o'ta toza stol yuzasini spirt bilan artishni, chiqindi suyuqlik va axlatni tozalashni unutmang.
Suyuqlikni o'zgartirganda, butun jarayon davomida sterillikka e'tibor bering. Operatorlar steril kiyim, steril qo'lqop va niqob kiyishlari kerak va ifloslanishni oldini olish uchun hujayra madaniyati kolbalari og'ziga qo'l bilan tegizishda ehtiyot bo'lishlari kerak.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.