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2. Materiallar va usullar
2.1. Huh7.5 Cells
Huh7.5 hujayra madaniyati saqlanishi hujayra madaniyati T flasks zardobida bo'lgan muhitda (SCM): Dulbecco's Modified Eagle Medium (DMEM) (Gibco, Peysley, UK) 4 mM GlutaMAX o'z ichiga olgan va bilan to'ldirilgan. 10% homila sigir zardobi (Sigma, Sent-Luis, MO, AQSh), 100 U/ml penitsillin va 100 mkg/ml streptomitsin (Sigma). T175 kolbalarida (Nunc, Roskilde, Daniya) va uch qatlamli kulturalarda (Nunc), Huh7,5 hujayralar har 2-3 kunda o'tkazildi. Barcha hujayra madaniyati 37 °C va 5% CO2 da saqlangan.
2.2. HCV virusi zahiralari
Ushbu tadqiqotda T flakonlarda bir qatlamli Huh7.5 hujayra kulturalarida yetishtirilgan 5a rekombinant SA13/JFH1core-NS5B genotipining ketma-ketlik bilan tasdiqlangan ikkita zaxirasi ishlatilgan. Zardobli (SC) sharoitlarda yetishtirilgan 5,8 log10 FFU/ml yuqumlilik titriga ega uchinchi o‘tish virusli zahiradan [39] CelCradle™ kulturalarini emlash, kolba kulturalarini silkitish va virus xarakteristikasi tajribalari uchun foydalanilgan. SF sharoitida yetishtirilgan 5,1 log10 FFU/ml [32] infektsion titri [39] boʻlgan toʻrtinchi oʻtishli virus zaxirasi virusni tavsiflash tajribalari uchun ishlatilgan.
Manba: https://www.mdpi .com/2076-393X/10/2/249/htm
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.