English
dagi hujayralarni etishtirishda mikoplazmaning ifloslanishi juda keng tarqalgan muammo hisoblanadi. Boshqa ifloslanishlardan farqli o'laroq, mikoplazma bilan ifloslangan hujayralar odatda bulutli bo'lmaydi, shuning uchun yalang'och ko'z bilan hukm qilish qiyin. Hujayralarning mikoplazma bilan ifloslanganligini aniqlashning to'rtta usuli mavjud:1. DNK floresan bo'yash hujayra fabrikalari
DNK floresan bo'yash usuli floresan bo'yog'i Hoechst33258 mikoplazma DNKsidagi AT asosiga boy hudud bilan bog'lanishi mumkin degan tamoyilga asoslanadi. Floresan nuqtalar AT asos mintaqalariga boy mikoplazma DNKsidir.
2. PCR texnologiyasi
Maxsus primerlar Mycoplasma genomidagi saqlanib qolgan ketma-ketliklarga muvofiq ishlab chiqilgan, tekshirilayotgan namunaning nuklein kislotasi kuchaytiriladi va tashxis kuchaytirilgan mahsulot hajmini tahlil qilish orqali amalga oshiriladi. PCR aniqlash texnologiyasi mikoplazma kontaminatsiyasini aniqlash uchun ishlatiladi, qisqa sikl, yuqori sezuvchanlik, yaxshi o'ziga xoslik, oddiy operatsiya va bir vaqtning o'zida ko'p miqdordagi namunalarni aniqlay oladi.
3. Ferment bilan bog'langan immunosorbent tahlili (ELISA)
ELISA mikoplazmaning ifloslanishini aniqlashda yaxshi o'ziga xoslik va sezgirlik bilan qo'llaniladi va bir vaqtning o'zida ko'p miqdordagi namunalarni aniqlashni yakunlashi mumkin. U oddiy, tez va miqdoriy sifat jihatidan aniqlash xususiyatlariga ega.
4. Elektron mikroskop
Odatda hujayra zavodlarida hujayralar 48 dan 72 soatgacha yetishtiriladi. Hujayralar deyarli birlashmasidan oldin, hujayra suspenziyasini hosil qilish uchun hujayralar tripsin bilan hazm qilinadi, mahkamlanadi, ko'miladi va kuzatishdan oldin dilimlanadi.
Yuqoridagilar hujayra fabrikalarida mikoplazma ifloslanishini aniqlashda keng tarqalgan to'rtta aniqlash usulidir. Hozirgi vaqtda hujayralarni ifloslantiruvchi mikoplazmaning 20 dan ortiq turlari ma'lum bo'lib, ularning 95% dan ortig'i og'iz mikoplazmasi, shuning uchun operatorlar hujayralarni etishtirishda aseptik operatsiyaga e'tibor berishlari kerak.
The above are four commonly used detection methods when detecting mycoplasma contamination in cell factories. At present, there are more than 20 kinds of mycoplasma known to contaminate cells, of which more than 95% are oral mycoplasma, so operators should pay attention to aseptic operation when culturing cells.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.