English
hujayra madaniyati sarf materiallarining ko'p qatlamli tuzilishi. Umumiy spetsifikatsiyalar 1 qatlam, 2 qatlam, 5 qatlam, 10 qatlam, 40 qatlam va boshqalarni o'z ichiga oladi. Qatlamlar soni qanchalik ko'p bo'lsa, madaniyat maydoni shunchalik katta bo'ladi. Ushbu sarf material asosan hujayra o'sishi uchun quyidagi shartlar zarur bo'lgan yopishgan hujayralar madaniyati uchun ishlatiladi:1. hujayra fabrikasi
Sterile environmentToksiklik va bepushtlik hujayra zavodlarida hujayralarni etishtirish uchun muhim shartlardir. In vivo, detoksifikatsiya tizimi va immunitet tizimi mikroorganizmlar yoki boshqa zararli moddalarning kirib kelishiga qarshi turishi mumkin, ammo in vitro madaniyati jarayonida hujayralar tananing immunitet tizimini himoya qila olmaydi va mikroorganizmlardan himoyalanish va zararli moddalarni zararsizlantirish qobiliyatini yo'qotadi. . Hujayralar in vitro muhitda o'sishi va ko'payishini ta'minlash uchun steril ish joyini, yaxshi shaxsiy gigienani, steril reagentlar va vositalarni va aseptik ishlov berishni ta'minlash kerak.
2. To'g'ri harorat
Umuman olganda, sut emizuvchilar va parrandalar hujayralarini in vitroda etishtirish uchun mos harorat 37-38 ° S dir. Noto'g'ri atrof-muhit harorati hujayralar o'sishiga ta'sir qiladi. Hujayralarning past haroratga chidamliligi yuqori haroratga qaraganda kuchliroqdir. Past haroratda hujayralarning metabolik faolligi va mitotik qobiliyati pasayadi. Harorat 0 ° C dan past bo'lmasa, hujayra metabolizmi ta'sirlangan bo'lsa-da, hech qanday zarar yo'q; 25 dan 35 ° C gacha, hujayralar sekin o'sadi; ammo 40 °C haroratda bir necha soat davomida joylashtirilsa, u nafaqat hujayraning omon qolishi va o'sishi uchun noqulay. , va hatto uning o'limiga olib keladi.
3. Tegishli osmotik bosim
Gipertonik yoki gipotonik eritmalar hujayralarning buklanishi, shishishi va yorilishiga olib kelishi mumkin. Shuning uchun osmotik bosim hujayralarni in vitroda etishtirishning muhim shartlaridan biridir. In vitro ekilgan hujayralarning ko'pchiligi osmotik bosimga ma'lum bardoshlik xususiyatiga ega. Amaliy ilovalarda ko'pchilik hujayralarga 260-320 mmol/L osmotik bosim qo'llanilishi mumkin.
4. Gaz muhiti va pH
Hujayralarning in vitro madaniyati ideal gaz muhitini talab qiladi. Kislorod va karbonat angidrid hujayraning omon qolishi uchun zarur shartdir. Kislorod hujayralarning trikarboksilik kislota aylanishida ishtirok etib, hujayraning yashashi, metabolizmi va sintezi uchun energiya beradi; karbonat angidrid nafaqat hujayralar metaboliti, hujayra o'sishi uchun muhim komponent, balki madaniyat muhitining pH darajasini saqlab qolish bilan ham bog'liq. Ko'pgina hujayralar uchun mos pH diapazoni odatda 7,2 dan 7,4 gacha. Ochiq madaniyatda 5% karbonat angidrid gazining nisbati mos keladi.
Yuqoridagilar hujayra zavodlarida hujayralarni etishtirish uchun to'rtta asosiy shartdir. Bu shartlar ekologik hisoblanadi. Bundan tashqari, hujayra o'sishi, shuningdek, hujayra o'sishi uchun zarur bo'lgan sarum, madaniyat muhiti va boshqa oziq moddalarni qo'shishi kerak, bu ham normal hujayra o'sishini ta'minlash uchun asosiy omil hisoblanadi.
The above are the four major conditions for culturing cells in cell factories. These conditions are environmental. In addition, cell growth also needs to add serum, culture medium and other nutrients required for cell growth, which is also a key factor to ensure normal cell growth.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.