Hujayra fabrikalarining sitotoksikligini tekshirish

Wed May 18 15:28:40 CST 2022

Sitotoksiklik - bu hujayra o'limi mexanizmi sifatida apoptoz yoki nekrozga tayanmaydigan hujayralar yoki kimyoviy moddalar tufayli yuzaga keladigan sof hujayralarni o'ldiradigan hodisa. Ushbu test orqali hujayra fabrikasi xom ashyosida hujayra o'sishiga yordam bermaydigan moddalar bor yoki yo'qligini aniqlash mumkin.

Sitotoksiklik - bu kimyoviy moddalarning (dorilarning) asosiy tuzilishga ta'siri va Hujayralarning fiziologik jarayoni, masalan, hujayra membranasi yoki sitoskeleton tuzilishi, hujayra metabolizmi jarayoni, hujayra tarkibiy qismlari yoki mahsulotlarining sintezi, degradatsiyasi yoki chiqarilishi, ionlarning regulyatsiyasi va hujayra bo'linishi va hujayraning yashashi, ko'payishi va/yoki buzilishiga olib keladigan boshqa jarayonlar funktsiyasi, natijada salbiy reaktsiyalar. Odatda, hujayra zavodining sitotoksikligini tekshirish quyidagi usullar bilan amalga oshirilishi mumkin:

1. MTT va XTT usullari: mitoxondriya ichidagi fermentlarning faolligidan foydalanib, o'ziga xos tetrazolium tuzlarini aylantirish mumkin, so'ngra mikroplata o'quvchi tomonidan aniqlanishi mumkin;

2. LDH usuli: sitotoksiklik hujayra madaniyati supernatantida LDH ferment faolligini aniqlash orqali aniqlanadi;

3. Boshqa enzimatik usullar: ishqoriy fosfataza va kislota fosfatazasining supernatantdagi faolligini aniqlash;

4. Hujayra proliferatsiyasini tahlil qilish to'plami: Oddiy hujayralar kuchli metabolizmga ega va ularning mitoxondriyalarida suksinat dehidrogenaza tetrazolium tuzlarini (MTT, XTT, WST-1 va boshqalar) hujayralar atrofida to'plangan binafsha rangli kristalli moddalarga kamaytirishi mumkin va keyin ODni o'qing. hujayra proliferatsiya holatini aniqlash uchun mikroplata o'quvchi tomonidan qiymat.

5. Flüoresan luminesans usuli bilan hujayra hayotiyligini aniqlash: adenilat kinaz (AK) barcha eukaryotik va prokaryotik hujayralar sitoplazmasida mavjud va AK ATPni hosil qilish uchun ADP ni faollashtirishi mumkin. Hujayralar shikastlanganda hujayra membranasi shikastlanadi va AK kultura supernatantiga chiqariladi. To'plam ATP ta'sirida yorug'lik chiqarish uchun lyusiferaza va lyusiferindan foydalanadi va uni xemiluminesans asbobi yordamida miqdoriy jihatdan aniqlash mumkin.

Hujayra zavodida sitotoksisite bor-yo'qligini tekshirish bir necha usulda o'tkazilishi mumkin va bu test ham quyidagilardan biridir. hujayra madaniyati sarf materiallari sifatini ta'minlash uchun muntazam sinov elementlari.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.