English
Lentivirus o'ziga xos retrovirus bo'lgan odamning immunitet tanqisligi virusidan (OIV) o'zgartirilgan virusli vektor turidir. In vitro tajribalari va in vivo tajribalarida lentivirus ekzogen genlar yoki ekzogen shRNKni ifodalash uchun vektorlarning keng tarqalgan shakllaridan biriga aylandi va tobora kengroq foydalanilmoqda. Ommaviy ishlab chiqarishda hujayra fabrikasi keng tarqalgan bo'lib foydalaniladigan hujayra madaniyati iste'mol qilinadi.
Lentiviral vektorlar maqsadni doimiy ifodalash effektiga erishish uchun ekzogen genlar yoki ekzogen shRNKlarni xost xromosomasiga samarali integratsiyalashi mumkin. ketma-ketliklar. Yuqumlilik nuqtai nazaridan u neyron hujayralarini, gepatotsitlarni, kardiyomiyositlarni, o'simta hujayralarini, endotelial hujayralarni, ildiz hujayralarini va boshqa turdagi hujayralarni samarali tarzda yuqtirishi mumkin, shuning uchun yaxshi gen terapiyasi ta'siriga erishish mumkin. Birlamchi hujayralar, ildiz hujayralari, ajratilmagan hujayralar va boshqalar kabi transfektsiyasi qiyin bo'lgan ba'zi hujayralar uchun lentiviral vektorlardan foydalanish maqsadli gen yoki maqsadli shRNKning transduksiya samaradorligini sezilarli darajada oshirishi mumkin va maqsadli gen yoki maqsadli shRNK mezbon hujayraga birlashtiriladi. Genomning ehtimoli sezilarli darajada oshadi va maqsadli gen yoki maqsadli shRNKning uzoq muddatli va barqaror ifodasi yanada qulayroq va tezroq amalga oshirilishi mumkin.
Hujayra zavodi asosan lentiviral vektorlarni kengaytirilgan ishlab chiqarishga qo'llaniladi. U hujayra madaniyatining sirt maydonini oshirishi mumkin, shu bilan vektorning rentabelligini oshiradi, transfeksiya va transfeksiyadan keyingi vositani almashtirish vaqtini kamaytiradi va mehnat xarajatlarini kamaytiradi. Bu samarali va qulay hujayra madaniyati sarflanadi.
Lentiviral vektorlarni keng miqyosda ishlab chiqarishda qo'llanilishidan tashqari, hujayra zavodlari hujayra terapiyasi, gen terapiyasi, onkolitik virus terapiyasining keng miqyosli ishlab chiqarishida muhim rol o'ynaydi. , va vaktsinalar.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.