English
Hujayra kultura plitasi biologik vositaning bir turi hisoblanadi. Shakliga ko'ra tekis tub va dumaloq tubga bo'linadi. Quduqlar soni 6 ta quduq, 12 ta quduq, 24 ta quduq, 48 ta quduq, 96 ta quduq, 384 ta quduq va boshqalarni oʻz ichiga oladi. U asosan hujayra madaniyati uchun ishlatiladi va oʻlchash uchun ham foydalanish mumkin. oqsil kontsentratsiyasi, 96-quduqli plitalar MTTni aniqlash uchun keng tarqalgan bo'lib qo'llaniladi.
MTT usuli, shuningdek, MTT kolorimetrik usuli deb ham ataladi, hujayraning omon qolishi va o'sishini aniqlash usulidir. Aniqlash printsipi shundaki, tirik hujayralar mitoxondriyalaridagi suksinat dehidrogenaza ekzogen MTT ni suvda erimaydigan ko'k-binafsha kristall formazanga (Formazan) kamaytirishi va uni hujayralarga joylashtirishi mumkin, o'lik hujayralar esa bunday funktsiyaga ega emas. Dimetil sulfoksid (DMSO) hujayralardagi formazanni eritishi mumkin va uning yorug'lik yutilish qiymati 570nm da ferment bilan bog'liq immunoassay bilan o'lchanadi, bu bilvosita tirik hujayralar sonini aks ettirishi mumkin. Hujayra raqamlarining ma'lum diapazonida MTT kristalining hosil bo'lishi miqdori hujayralar soniga proportsionaldir. Aniqlash bosqichlari quyidagicha:
1.
Hujayralarni 96-quduqli hujayra madaniyati plitasi bilan o'stirish. Hujayra zichligi mos bo'lgandan so'ng, har bir quduqqa 50ul MTT qo'shing va 4 soat davomida madaniyatni davom ettiring;
2.
Tarkibida MTT bo'lgan madaniy muhitni so'rib oling va 150ul DMSO qo'shing. Kristallarni to'liq eritish uchun gorizontal ravishda 10 daqiqa tebranish. To'xtatilgan hujayralar uchun, santrifüjdan so'ng, muhitni so'rib oling va keyin eritish uchun DMSO qo'shing;
3. Ferment bilan bog'langan immunoassay asbobida 550nm da absorbansni o'lchang (oqilona nazorat guruhi va bo'sh guruhni o'rnating);
4. Hujayra omon qolish darajasi=tajriba guruhining so'rilish qiymati/nazorat guruhining absorbsiya qiymati*100%.
Operatsiya davomida MTT kanserogen ekanligini ta'kidlash kerak. Iloji bo'lsa, shaffof plyonkali qo'lqop kiyishga harakat qiling. Tayyorlangan MTT steril bo'lishi kerak. MTT bakteriyalarga juda sezgir. 96 quduqli hujayra madaniyati plastinkasini yorug'likdan himoya qilmasdan qo'shsangiz, bu muhim emas. , Axir, vaqt qisqa, yoki siz konsoldagi yoritishni o'chirib qo'yishingiz mumkin.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.