English
Sf9 hujayralari Spodoptera frugiperda hujayralari bo'lib, ular odatda turli dorivor oqsillar va viruslarni ishlab chiqarish yoki asosiy tadqiqotlar uchun ishlatiladi. Sf9 hujayralarini etishtirishda keng tarqalgan ishlatiladigan hujayra madaniyati sarf materiallari hujayra madaniyati flasks.
Sf9 hujayralar Qo'shma Shtatlar Qishloq xo'jaligi departamentining hasharotlar patologiyasi laboratoriyasidan olingan va IPLBSF-21 hujayrasidan olingan. chiziq. Hujayralar zarrachalar bilan muntazam bo'lib, suspenziya yoki yopishqoq kulturada etishtirilishi mumkin. Bu hujayra yuqori ekspressiya darajasiga ega (ayniqsa hujayra ichidagi oqsillar), tezroq o'sish tezligiga ega, oqsilning samarali qatlamlanishi, glikozillanish sutemizuvchilar hujayralariga o'xshaydi va nisbatan oson enzimatik deglikozillanish (oqsil tuzilishini aniqlash uchun) Qulay), ifodalashda keng qo'llaniladi. ning V LPs.
Sf-9 hujayralari yarim yopishgan hujayra chiziqlaridir. 10% xomilalik buzoq zardobini o'z ichiga olgan muhitda hujayralarning aksariyati odatda yopishib o'sadi va kichik qismi to'xtatiladi. CO2 holda 27 ° C hujayra inkubatorida o'sadi. Odatda u zichligi 80-95% gacha o'sganda o'tish uchun pastki shisha bo'lishi mumkin. O'tish paytida tripsin hazm qilish talab qilinmaydi. 1 ml pipetka bilan yopishgan hujayralarni 5-10 marta yumshoq pipetlash orqali hujayralarni suyultirish va yopishmaganidan keyin o'tish mumkin. Hujayra madaniyati kolbalarining umumiy xususiyatlari 25cm2, 75cm2, 175cm2, 225cm2 va boshqalarni o'z ichiga oladi. TC bilan ishlov berilgan sirt yopishqoq hujayra madaniyati uchun mos keladi va TC bilan ishlov berilmagan sirt o'sish va madaniyatga javob beradigan suspenziya hujayra madaniyati uchun javob beradi. sf9 hujayralarining ehtiyojlari.
Hujayradan o'tish operatsiyalarini bajarayotganda, hujayra shikastlanishiga yo'l qo'ymaslik va hujayra madaniyati jarayoniga ta'sir qilish uchun hujayra madaniyati kolbalarini kuchli silkitmaslik tavsiya etiladi.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.