English
Hujayra kulturalariturli shakllarga ko'ra tekis bo'yin, tortikollis, burchakli bo'yin, uchburchak, to'rtburchak va boshqalarga ega. Texnik xususiyatlarga 25ml, 75ml, 175ml va 250ml kiradi. Kichikroq o'lchamlar asosan hujayralarni o'tkazish, hujayralarni saqlash va tajribalar uchun hujayralar bilan ta'minlash va hokazolar uchun ishlatiladi.
Hujayralar hujayra madaniyati kolbasida zich monoqatlamga aylangandan so'ng, ular asosan to'yingan. Hujayralarning o'sishda davom etishi va hujayralar sonini ko'paytirishi uchun ularni subkulturalash (qayta etishtirish) kerak. Subkultura ham hujayra turlarini saqlab qolish usulidir. Shu bilan birga, bu madaniy hujayralar yordamida turli tajribalar uchun zarur jarayondir. To'xtatilgan hujayralarni to'g'ridan-to'g'ri ajratish mumkin, yopishgan hujayralarni ajratishdan oldin hazm qilish kerak. Muayyan qadamlar quyidagilardan iborat:
1. Asl madaniyat muhitini hujayralar bilan to'ldirilgan hujayra madaniyati kolbasiga tashlang.
2. 0,5-1ml 0,25% li pankreatin eritmasi qo'shing, shunda shishaning pastki qismidagi hujayralar probirkaga botiriladi.
3. Shishaga rezina tiqin qo'ying va hujayralarni kuzatish uchun uni teskari mikroskop ostiga qo'ying. Vaqt o'tishi bilan asl yopishgan hujayralar asta-sekin yumaloq bo'lishga moyil bo'lib, hujayralar hali suzmaganida, pankreatin tashlab yuborildi va ovqat hazm qilishni tugatish uchun 10 ml madaniy muhit qo'shildi. Ovqat hazm qilishni kuzatish yalang'och ko'z bilan ham amalga oshirilishi mumkin. Shishaning pastki qismida oq rang paydo bo'lganda va mayda teshiklar paydo bo'lganda, ovqat hazm qilish to'xtatilishi mumkin. Odatda xona haroratida hazm qilish vaqti taxminan 1-3 minut.
4. Pipetka yordamida yopishgan hujayralarni suspenziyaga puflang, ularni ikki-uch shishaga bo'ling, kultura eritmasini rezina tiqin bilan yopib qo'ying va kultivatsiyani davom ettirish uchun uni 37 ° C ga qo'ying. Ertasi kuni yopishgan o'sishni kuzating.
Yuqoridagilar hujayra o'tishida hujayra kulturalari ning qo'llanilishi. Ovqat hazm qilish eritmasini tayyorlash usuli quyidagicha: 0,25 g tripsinni torting (faoliyati 1:250), eritish uchun 100 ml Ca2+, Mg2+siz Hank eritmasini qo'shing, filtrlang Filtrlash orqali sterilizatsiya qiling, 4 ° C da saqlang va 37 ° C haroratga qayting. Ishlatishdan oldin °C. Yakuniy konsentratsiyani 0,02% ga yetkazish uchun EDTA pankreatin eritmasiga ham qo‘shilishi mumkin.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.