English
yopishtiruvchi hujayra madaniyati uchun tez-tez ishlatiladigan sarf materialidir. Tajriba davomida biz hujayralarning o'sish holatini real vaqtda tushunishimiz va muayyan vaziyatga qarab tegishli tuzatishlar kiritishimiz kerak. Hujayra o'sishini aniqlash uchun hujayra hayotiyligini MTT usuli bilan aniqlash mumkin.MTT usuli, shuningdek, MTT kolorimetrik usuli sifatida ham tanilgan, hujayraning omon qolishi va o'sishini aniqlash usulidir. Aniqlash printsipi shundan iboratki, tirik hujayralar mitoxondriyalaridagi suksinat dehidrogenaza ekzogen MTTni suvda erimaydigan ko'k-binafsha rangli kristalli formazanga kamaytirishi va hujayralardagi cho'kishi mumkin, o'lik hujayralar esa bunday funktsiyaga ega emas. Dimetil sulfoksid (DMSO) hujayralardagi formazanni eritishi mumkin va uning yorug'lik yutilish qiymati 570 nm to'lqin uzunligida ferment bilan bog'langan immunosorbent tahlili bilan o'lchanishi mumkin, bu bilvosita tirik hujayralar sonini aks ettirishi mumkin. Muayyan hujayralar soni oralig'ida MTT kristalining hosil bo'lishi miqdori hujayra soniga mutanosibdir. Ushbu usul ba'zi biologik faol omillarning faolligini aniqlashda, keng ko'lamli o'simtaga qarshi dori skriningida, sitotoksiklik testida va o'simta radiosensitivligini tahlil qilishda keng qo'llanilgan. U yuqori sezuvchanlik va tejamkorlik bilan ajralib turadi.hujayra fabrikasi
Hujayra zavodida hujayralarning hayotiyligini tekshirishdan oldin, MTTni shakllantirish juda muhim bosqichdir. Odatda, bu usulda MTT konsentratsiyasi 5 mg / ml ni tashkil qiladi. Shuning uchun 0,5 g MTTni tortish mumkin, 100 ml fosfat buferli sho'r suvda (PBS) yoki fenol qizilsiz muhitda eritiladi, probirkadagi bakteriyalarni yo'qotish uchun 0,22 mkm filtr bilan filtrlanadi va qorong'i joyda 4 ° C haroratda saqlanadi. . . Tayyorlash va saqlash vaqtida idishni alyuminiy folga bilan o‘rashga harakat qiling.
Xujayra zavodida hujayra faolligini o‘lchash uchun MTT usulidan foydalanilganda, MTT kanserogen ekanligini ta’kidlash kerak, shuning uchun uni ishlatishda ehtiyot bo‘ling. Bu usul faqat hujayraning nisbiy soni va nisbiy hayotiyligini aniqlash uchun ishlatilishi mumkin, lekin mutlaq hujayra soni emas. Natijalarni mikroplastinka o'quvchi bilan sinovdan o'tkazishda, tajriba natijalarining chiziqliligini ta'minlash uchun MTT absorbansi maksimal darajada 0-0,7 oralig'ida bo'lishi kerak.
When using the MTT method to measure the cell activity in the cell factory, it should be noted that MTT is carcinogenic, so be careful when using it. This method can only be used to detect relative cell number and relative viability, but not absolute cell number. When testing the results with a microplate reader, in order to ensure the linearity of the experimental results, the MTT absorbance should be in the range of 0-0.7 as much as possible.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.