English
2. Material och metoder
2.1. Underhåll av Huh7.5 Cells
Huh7.5 cellkulturer hölls i cellodlings-T-kolvar i seruminnehållande medium (SCM): Dulbecco's Modified Eagle Medium (DMEM) (Gibco, Paisley, Storbritannien) innehållande 4 mM GlutaMAX och kompletterat med 10 % fetalt bovint serum (Sigma, St. Louis, MO, USA), 100 U/ml penicillin och 100 µg/ml streptomycin (Sigma). I T175-kolvar (Nunc, Roskilde, Danmark) och trippelskiktsodlingskolvar (Nunc) passerades Huh7.5-celler varannan till var tredje dag. Alla cellkulturer hölls vid 37 °C och 5 % CO2.
2.2. HCV Virus Stocks
Två sekvensbekräftade stammar av genotyp 5a rekombinant SA13/JFH1core-NS5B [28], odlade i monolager Huh7.5 cellkulturer i T-kolvar, användes i denna studie. En tredje passage virusstam med en infektivitetstiter på 5,8 log10 FFU/ml, odlad under seruminnehållande (SC) förhållanden [39], användes för att ympa CelCradle™-kulturer, skaka kolvkulturer och för viruskarakteriseringsexperiment. En fjärde passage-virusstam med en smittsamhetstiter på 5,1 log10 FFU/mL [32], odlad under SF-förhållanden [39], användes för viruskarakteriseringsexperiment.
Källa från: https://www.mdpi .com/2076-393X/10/2/249/htm
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.