English
S poglobljenim razvojem področja znanosti o življenju se tehnologija celične kulture široko uporablja na številnih področjih, kot so biofarmacevtika, industrijska proizvodnja cepiv in monoklonska protitelesa. Glede na različne načine rasti celic se deli v dve kategoriji: adherentne celice in suspenzijske celice. Katere celice je torej mogoče gojiti z uporabo cell factories?
Tovarna celic je večplastna struktura potrošnega materiala celične kulture. Skupne specifikacije vključujejo 1 plast, 2 plasti, 5 plasti, 10 plasti, 40 plasti itd. Uporablja se predvsem za kulturo adherentnih celic. Rast adherentnih celic mora imeti podporno površino, ki jo je mogoče pritrditi, celice pa lahko rastejo in se razmnožujejo na tej površini, tako da se zanašajo na faktorje pritrditve, ki jih izločajo sami ali so na voljo v gojišču. Ko se celice pritrdijo, običajno v nekaj dneh prekrijejo površino kulture in tvorijo gosto celično monoplast, kot so celice Vero, celice HEK 293, celice CAR-T, celice MRC5, celice CEF, prašičji alveolarni makrofagi, celice mieloma, DF - 1 celice, ST celice, celice PK15, celice Marc145 itd. so bile vse prilepljene na celično tovarno za kulturo.
Poleg tega se lahko ta potrošni material uporablja tudi za stacionarno kulturo suspenzijskih celic. Suspenzivne celice se nanašajo na celice, ki rastejo neodvisno od površine nosilca in rastejo v suspenziji v gojišču, kot so limfociti.
Tovarne celic se običajno proizvajajo iz polistirenskih surovin, površina pa je hidrofobna. Če se uporablja za adherentno celično kulturo, je treba površino obdelati s TC, da se poveča njena hidrofilnost, ki je primerna za rast celic.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.