English
Združevanje celic je pogosta težava pri uporabi bučke za celično kulturo za celično kulturo. Združevanje celic bo vplivalo na normalno rast in razmnoževanje celic. Kako potem rešiti ta problem?
Najprej, združevanje celic ne vpliva nujno na proces celične kulture. Če ima celica jasen obris in prepustno celično telo, ki je enotno združeno, to dokazuje, da celica dobro raste. Vendar pa imajo v večini primerov gručaste celice mehke obrise, slabo prepustnost svetlobe in celo sincicijo, ki jo spremljajo celični ostanki, kar dokazuje, da so celice stare ali obolele in so v slabem stanju. Obstaja več načinov za rešitev problema združevanja celic:
1. Če gruščanje celic ne vpliva na rast, ni problema, vendar je štetje bolj težavno. Med prebavo, preden se celice spremenijo iz poligonalne oblike v okroglo, z roko potrkajte ob stransko steno bučke s celično kulturo, da celice odpadejo s stene (najbolje je, da ne pihate, kar ni dobro za oblika in rast celic). Za
2. Če začutite DNK mrtvih celic, dodajte nekaj kapljic sterilne DNAze (1 mg/ml, raztopljene v vodi) v celično suspenzijo, da prekinete verigo DNK. Nežno prepihajte celice, da preprečite fizično poškodbo celične membrane.
3. Če so celične kepe vidne s prostim očesom, pustite celice stati približno pol minute, nato pa odpipetirajte zgornjo polovico celične suspenzije brez grudic za prehod. Če celične grude niso vidne s prostim očesom, trenutno ni posebej dobre metode ločevanja in te celice je mogoče počasi odstraniti šele, ko se stanje celic izboljša. se lahko zdravi po zgornjih metodah. Med procesom celične kulture je treba pravočasno opazovati stanje rasti celic in narediti ustrezne prilagoditve glede na situacijo.
If the cells in the cell culture flasks appear clumping, they can be treated according to the above methods. During the cell culture process, it is necessary to observe the growth status of the cells in time and make corresponding adjustments according to the situation.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.