English
Cell factoriesse uporabljajo predvsem za obsežno kulturo adherentnih celic, kot so cepivo proti hepatitisu A, cepivo proti hepatitisu B, cepivo proti noricam itd. Pri gojenju celic včasih ugotovimo, da se celice težko oprimejo stene. Kaj je razlog?
Najprej obstaja veliko razlogov za pojav neadherence pri gojenju celic v celični tovarni, ki jih je mogoče analizirati in rešiti z naslednjih vidikov:
Prekomerna prebava tripsina: tripsin pomaga celicam pri prebavi. Če je prebava pretirana, bo aktivnost celic močno poškodovana in celice bodo lebdile. To je mogoče nadzorovati s skrajšanjem časa prebave ali znižanjem koncentracije tripsina.
kontaminacija z mikoplazmo: celice so občutljive na okolje. Če upravljavec ne pazi na higieno ali pa sta delovno okolje in eksperimentalna oprema kontaminirana, bo to povzročilo navzkrižno kontaminacijo med celicami in pojav nelepljenja. Če ugotovite kontaminacijo z mikoplazmo, kulturo nemudoma zavrzite.
Celično staranje: Staranje celic je vsota degenerativnih sprememb v celicah, ko se organizem stara, zaradi česar celice izgubijo adhezijo, če se zlijejo pred prehodom. Sveže posejane celice lahko odtajamo in ponovno gojimo.
Poleg tega, če je začetna koncentracija inokuliranih celic prenizka ali previsoka, bo to vplivalo tudi na lastnosti adhezije celic, prilagajanje ustrezne koncentracije inokuliranih celic pa lahko reši ta problem. Pred uporabo tovarne celic morajo operaterji dobro razkužiti sebe in okolje, da se izognejo različnim kontaminacijam, ki vplivajo na proces celične kulture.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.