English
Kontaminacija z mikoplazmo je pogost problem v temeljnih raziskavah in industrijski proizvodnji. Pri uporabi cell factories za celično kulturo ali obsežno industrijsko proizvodnjo je zelo moteča tudi kontaminacija z mikoplazmo. Kaj torej storiti v tej situaciji?
Mikoplazma je mikroorganizem, katerega velikost je med bakterijo in virusom (minimalni premer 0,2 um) in živi samostojno. Ko mikoplazma kontaminira celice, gojišče morda ne postane motno. Razbremeni se s prehodom in izmenjavo medija, zato ga je enostavno prezreti. Toda nekateri resni primeri lahko povzročijo počasno proliferacijo celic in celo odpadejo iz tovarne celic.
Če pride do kontaminacije z mikoplazmo, za nepomembne celice, kot so celice v kulturi, celice WCB itd., kultura in uporabljeni medij je mogoče inaktivirati in zavreči. Za pomembnejše celice lahko za zdravljenje uporabimo zdravljenje z MRA, zdravljenje s segrevanjem s pomočjo zdravil, uporabo seruma, specifičnega za mikoplazmo, inokulacijo in sterilizacijo pri živalih, fagocitozo makrofagov in uporabo medija za čiščenje mikoplazme.
Preprečevanje mikoplazme. kontaminacija je ključna pri uporabi celičnih tovarn za delo na celični kulturi. Verjetnost kontaminacije z mikoplazmo je mogoče zmanjšati z nadzorom onesnaženosti okolja, strogim eksperimentalnim delovanjem, zagotavljanjem sterilnosti medija in opreme celične kulture ter dodajanjem ustrezne količine antibiotikov v medij celične kulture.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.