Kakšne vrste celic je mogoče gojiti v bučkah za celične kulture?

Bučke za celično kulturose večinoma uporabljajo za majhne in srednje celične kulture, torej katere vrste celic je mogoče gojiti v bučkah za celično kulturo?

Obstaja veliko vrst celic, ki so razdeljene na adherentne celice in suspenzijske celice glede na njihove vzorce rasti. Rast adherentnih celic mora imeti podporno površino, na katero se lahko pritrdi, celice pa se zanašajo na pritrdilne faktorje, ki jih izločajo same ali so na voljo v gojišču. Za rast in razmnoževanje na tej površini. Rast suspenzijskih celic ni odvisna od površine nosilca in raste v suspenziji v gojišču. Bučke za celične kulture so običajno izdelane iz prozornih polistirenskih surovin. Skupne specifikacije vključujejo 25cm2, 75cm2, 175cm2 in 225cm2. Spodnja površina celične bučke je oblikovana v obliki loka za zmanjšanje mrtvih kontaktnih kotov pri pipetiranju celic. Uporablja se lahko tako za kulturo adherentnih celic kot za statično kulturo suspenzijskih celic. Veljavne vrste celic so naslednje:

Adherentne celice: celice Vero, celice HEK 293, celice CAR-T, MRC5, celice CEF, prašičji alveolarni makrofagi, celice mieloma, celice DF-1, celice ST, celice PK15, Marc145 celice itd.

Celice suspenzije: celice CHO, celice žuželk, celice BHK21 in celice MDCK itd.

Opozoriti je treba, da če se bučke za celično kulturo uporabljajo za gojenje adherentnih celic, površina bučko je treba posebej modificirati, da se poveča njena hidrofilnost, da ustreza rastnim značilnostim adherentnih celic. Pri uporabi v suspenzijski celični kulturi ni potrebna posebna obdelava.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.