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je neke vrste potrošni material za celično kulturo tipa steklenice. Za spodbujanje učinkovitosti oprijema celic so potrošni materiali prevlečeni pri gojenju primarnih celic. Običajno uporabljen pufer za prevleko vključuje naslednje:polilizin: je sintetična spojina, pozitivno nabita aminokislina, ki spodbuja celično adhezijo s spremembo elektrode na površini substrata kulture. Široko se uporablja v celični kulturi za premaze za spodbujanje celične adhezije. Poleg spodbujanja celične adhezije in rasti lahko polilizinska prevleka z raztopino za prevleko poveča stopnjo preživetja primarnih celic in spodbuja razraščanje nevritov.bučka za celično kulturo
Fibronektin: je visokomolekularni glikoprotein, ki so ga začeli raziskovati in odkrivati v tujini leta 1974 in ima različne biološke funkcije. Fibronektin je glikoprotein z molekulsko maso 440-500 kDa. Najdemo ga v beljakovinah celične površine in plazmi. Združuje se s površinskimi receptorji celične membrane in komponentami zunajceličnega matriksa za spodbujanje celične adhezije in rasti razširitev.
Želatina: mešanica vodotopnih beljakovin z visoko molekulsko maso, pridobljenih iz kolagena, 0,2 % tekoča raztopina želatine za prevleko se uporablja za prevleko posod kulture za spodbujanje celične adhezije. Predstavite človeške embrionalne matične celice za diferenciacijo, nekatere primarne celice in ovekovečene celice.
Zgoraj je več puferjev za prevleko, ki se običajno uporabljajo v bučkah za celične kulture. Posebna izbira pufra za premaz je odvisna od vrste in značilnosti celic. Vendar je treba opozoriti, da interval med premazom in uporabo steklenice ne sme presegati enega dneva. Dlje ko je prevlečena posoda shranjena, bo to vplivalo na njeno oprijemljivost.
The above are several coating buffer commonly used in cell culture flasks. The specific choice of coating buffer depends on the type and characteristics of the cells. However, it should be noted that the interval between coating and use of the bottle should not exceed one day. The longer the coated container is stored, its adhesion performance will be affected.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.