English
Cell Factoryje potrošni material večplastne celične kulture, primeren za obsežno celično kulturo. Poleg specifičnega okolja za celično kulturo je bistvena rešitev tudi prebavni sok.
Pri in vitro kulturi tkivnih celic in disperziji tkivnih celic v primarni celični kulturi (pripravljen je tkivni blok v suspenzijo ene same celice), pa tudi pri subkulturi je treba za prebavo in razpršitev adherentnih rastočih celic uporabiti raztopino za prebavo tkivnih celic. Raztopina za prebavo, ki se uporablja v tehnologiji celične tovarne, je predvsem tripsin, EDTA itd.
Glavne sestavine celične membrane in adhezije med celicami so lipoproteini in polisaharidi. Tripsin v tripsinu lahko pretvori beljakovine v peptone, lipaza trebušne slinavke pa lahko razgradi maščobo v glicerol in maščobne kisline ter tako loči celice. Celice se lizirajo. Naloga raztopine za prebavo je predvsem hidroliziranje medceličnih beljakovin (kot je zunajcelični matriks), razpršitev tkiva ali adhezivnih celic v posamezne celice in priprava celične suspenzije za nadaljnje poskuse.
Prebavni encimi igrajo pomembno vlogo v kulturi celičnih tovarn, vendar je treba opozoriti, da imajo različne celice različne odzive na encime trebušne slinavke. Ko je pH vrednost 8,0 in temperatura 37 °C, ima najmočnejši učinek raztopina encimov trebušne slinavke. Pri uporabi je treba koncentracijo, temperaturo in čas dobro nadzorovati, da se izognemo prekomerni prebavi, ki bi vplivala na oprijem in rast celic.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.