English
je enoslojna ali večplastna struktura potrošnega materiala celične kulture. Med procesom celične kulture moramo pravočasno razumeti rastno stanje celic, preden nadaljujemo na naslednji korak. Število živih celic je mogoče zaznati z obarvanjem s tripan modro. Posebna metoda delovanja je naslednja:1. Za adhezivne celice odsesajte medij, dodajte raztopino za razgradnjo in prebavite v inkubatorju primeren čas. Ko so celice postale okrogle, smo prebavo zaključili z dodajanjem enakega ali večjega volumna medija od volumna digestata. Po centrifugiranju pripravimo celično suspenzijo z gojiščem;cell factory
2. Vzemite 0,5 ml raztopine tripan modrega, 0,3 ml PBS in 0,2 ml celične suspenzije in dobro premešajte. Nato zmes pustimo 1-2 minuti. S kapilarno pipeto ali 10-ul konico majhne pipete potegnite majhno količino mešanice in jo injicirajte v hemocitometer;
3. Preštejte vsaj 1000 celic in preštejte število obarvanih celic;
4. Operite štetno ploščo in pokrovno stekelce z ultra čisto vodo in 75 % etanolom;
5. Normalne celice ne bodo obarvane, šele potem, ko celice odmrejo, se prepustnost celične membrane poveča, tripan modro vstopi v celice in celice so videti modre. Mešanice ne smemo stati predolgo, sicer bodo barvilo prevzele tudi normalne žive celice, kar bo vplivalo na natančnost štetja;
6. Viabilnost celic (%) = (skupno število celic - število obarvanih celic)/skupno število celic*100;
7. Tako kot metoda štetja celic ima obarvanje s tripan modrim veliko napako. Zazna lahko le zgodnjo stopnjo apoptoze, ne more pa pokazati razlike med kontrolno in eksperimentalno skupino.
V procesu gojenja celic v celični tovarni vedno pride nekaj celic, ki umrejo zaradi različnih razlogov. Obvladovanje števila živih celic in sposobnosti preživetja celic je ključna operacija v poskusih s celično kulturo in učinkovit način za zagotavljanje napredka poskusa.
In the process of culturing cells in a cell factory, there are always some cells that die due to various reasons. Mastering the number of living cells and cell viability is a key operation in cell culture experiments and an effective way to ensure the progress of the experiment.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.