Kako narediti bučke s celično kulturo brez DNK in RNKaze

Celična kultura je nekakšna aseptična tehnologija delovanja, ki zahteva, da delovno okolje in pogoji ne smejo biti kontaminirani z mikrobi in nanje ne vplivajo drugi škodljivi dejavniki. Bučke za celične kulture so običajno uporabljeni potrošni materiali v poskusih s celicami. Odsotnost DNK in RNase sta osnovni zahtevi za potrošni material. Torej, kako so lahko bučke s celično kulturo brez DNK in RNaze?

Soba za celične kulture in načelo oblikovanja morata preprečiti mikrobno kontaminacijo in škodljive dejavnike ter zahtevata čisto delovno okolje, svež zrak, suhost in brez dima. Proizvodne delavnice za bučke za celične kulture in drugi potrošni material so vse delavnice GMP na 100.000 ravneh, proizvodne delavnice za zdravila za ljudi pa so tudi na 100.000 stopnjah. Zato izdelki, proizvedeni v takšnih pogojih, na splošno ne vsebujejo encimov DNK in RNA. In potem, ko bo končni izdelek prišel na trg, bo uporabil tudi poskuse razgradnje DNK in RNA za testiranje izdelka in samo kvalificirani izdelki lahko zapustijo tovarno.

Kvalificirane bučke za celične kulture so ključni dejavnik za zagotovitev rasti celic. Potrošni material in pripomočke, ki se uporabljajo v celični kulturi, je treba pravočasno avtoklavirati in ponovno sterilizirati, če jih ne uporabljate več kot en teden po sterilizaciji. Pripravljen popoln medij, pankreatin, PBS itd. je najbolje porabiti v enem tednu. Hkrati vsak dan preverite stanje celice. V celični sobi bi morali zmanjšati hojo in čim manj govoriti. Če kašljate ali kihate, ne pozabite obrniti hrbet ultra čisti mizi. Ohranite okoljsko higieno v laboratoriju in postavite temelje za uspeh celičnega eksperimenta.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.