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2. Materiali in metode
2.1. Vzdrževanje celičnih kultur Huh7.5
Huh7.5 smo vzdrževali v T bučke za celično kulturo v mediju, ki vsebuje serum (SCM): Dulbecco's Modified Eagle Medium (DMEM) (Gibco, Paisley, UK), ki vsebuje 4 mM GlutaMAX in dopolnjen z 10 % fetalni goveji serum (Sigma, St. Louis, MO, ZDA), 100 U/mL penicilina in 100 µg/mL streptomicina (Sigma). V bučkah T175 (Nunc, Roskilde, Danska) in troslojnih bučkah za kulturo (Nunc) so bile celice Huh7,5 pasirane vsake 2–3 dni. Vse celične kulture smo vzdrževali pri 37 °C in 5 % CO2.
2.2. V tej študiji sta bili uporabljeni dve zaporedji potrjeni staleži genotipa 5a rekombinantnega SA13/JFH1core-NS5B [28], gojeni v enoslojnih celičnih kulturah Huh7.5 v bučkah T. Zaloga virusa tretjega prehoda s titrom infektivnosti 5,8 log10 FFU/mL, gojena v pogojih, ki vsebujejo serum (SC) [39], je bila uporabljena za inokulacijo kultur CelCradle™, kultur stresanja bučk in za poskuse za karakterizacijo virusa. Za poskuse karakterizacije virusa smo uporabili zalogo virusa četrtega prehoda s titrom infektivnosti 5,1 log10 FFU/mL [32], vzgojeno v pogojih SF [39].
Vir iz:
https://www.mdpi .com/2076-393X/10/2/249/htmhttps://www.mdpi.com/2076-393X/10/2/249/htm
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.