English
Gojenje celic je zelo stroga naloga. Poleg ustvarjanja dobrega rastnega okolja za celice mora med procesom gojenja nemudoma reševati tudi različne nujne primere. Združevanje celic v bučke s celično kulturo je pojav, s katerim se pogosto srečujemo. Kaj je razlog za to situacijo?
Fenomen združevanja celic v bučkah s celično kulturo lahko na splošno analiziramo z naslednjih vidikov:
1. Proces prebave je lahko preveč grob, premočno pipetiranje, predolga prebava, premočan ali strupen prebavni sok itd., bo povzročilo smrt celic. Ko celica umre, sprosti DNK, zaplete druge celice in tvori lepljivo celično gručo.
2. Prevelika hitrost centrifugiranja celic ali predolg čas lahko prav tako zlahka povzročita grude celic.
3. Ko prebava ni končana, se povezava med celicami in celico ne loči; ko je prebava končana, se okrogle celice nabirajo in jih je težko ponovno ločiti.
4. Celice, ki so v slabem stanju in se starajo, lahko rastejo tudi v gručah (kot je slabo stanje celic, ki je posledica zamude pri menjavi medija, predolgega prehoda, onesnaženja itd.).
5. Pri prehodu ni enakomernega pipetiranja in je veliko celičnih grudic, ki bodo ob gojenju v gruči.
6. Neenakomerna inokulacija ali neprebavljene celice.
7. Kultura brez vibracij ali prevelika količina bakterij (celic) bo tvorila grude.
Če se celice v bučke za celično kulturo zdijo grude, lahko analizirate zgornje vidike, da ugotovite razlog, in pred operacijo oblikujete stroge postopke, da se izognete ta pojav.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.