English
2. Materiály a metódy
2.1. Udržiavanie buniek Huh7.5
Bunkové kultúry Huh7.5 sa udržiavali v V tejto štúdii sa použili dve zásobné vzorky rekombinantného SA13/JFH1core-NS5B genotypu 5a s potvrdenou sekvenciou [28], pestované v monovrstvových bunkových kultúrach Huh7.5 v T fľašiach. Tretia pasáž vírusového zásobného roztoku s titrom infekčnosti 5,8 log10 FFU/ml, pestovaná v podmienkach obsahujúcich sérum (SC) [39], sa použila na inokuláciu kultúr CelCradle™, kultúr v trepacích fľašiach a na experimenty na charakterizáciu vírusu. Na experimenty na charakterizáciu vírusu sa použila štvrtá pasáž vírusu s titrom infekčnosti 5,1 log10 FFU/ml [32], pestovaná v podmienkach SF [39]. v médiu obsahujúcom sérum (SCM): Dulbeccovo modifikované Eagle médium (DMEM) (Gibco, Paisley, UK) obsahujúce 4 mM GlutaMAX a doplnené o 10 % fetálne hovädzie sérum (Sigma, St. Louis, MO, USA), 100 U/ml penicilínu a 100 ug/ml streptomycínu (Sigma). V bankách T175 (Nunc, Roskilde, Dánsko) a trojvrstvových kultivačných bankách (Nunc) boli bunky Huh7.5 pasážované každé 2 až 3 dni. Všetky bunkové kultúry sa udržiavali pri 37 °C a 5 % C02. T banky bunkovej kultúry 2.2. Zásoby vírusu HCV
Zdroj:
https://www.mdpi .com/2076-393X/10/2/249/htm
Source from: https://www.mdpi.com/2076-393X/10/2/249/htm
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.