English
2. Materiais e Métodos
2.1. Manutenção de células Huh7.5 As culturas de células Huh7.5 foram mantidas em
em meio contendo soro (SCM): Dulbecco's Modified Eagle Medium (DMEM) (Gibco, Paisley, UK) contendo GlutaMAX 4 mM e suplementado com 10% de soro fetal bovino (Sigma, St. Louis, MO, EUA), 100 U/mL de penicilina e 100 µg/mL de estreptomicina (Sigma). Em frascos T175 (Nunc, Roskilde, Dinamarca) e frascos de cultura de camada tripla (Nunc), células Huh7.5 foram passadas a cada 2-3 dias. Todas as culturas celulares foram mantidas a 37°C e 5% de CO2. Frascos T de cultura celular 2.2. HCV Virus StocksFonte de: Duas sequências confirmadas do genótipo 5a recombinante SA13/JFH1core-NS5B [28], cultivadas em culturas de células Huh7.5 em monocamada em frascos T, foram usadas neste estudo. Um estoque de vírus de terceira passagem com um título de infectividade de 5,8 log10 FFU/mL, cultivado sob condições contendo soro (SC) [39], foi usado para inocular culturas CelCradle™, culturas em frascos de agitação e para experimentos de caracterização de vírus. Um estoque de vírus de quarta passagem com um título de infectividade de 5,1 log10 FFU/mL [32], cultivado sob condições de SF [39], foi usado para experimentos de caracterização de vírus.
https://www.mdpi .com/2076-393X/10/2/249/htm
Two sequence-confirmed stocks of the genotype 5a recombinant SA13/JFH1core-NS5B [28], grown in monolayer Huh7.5 cell cultures in T flasks, were used in this study. A third passage virus stock with an infectivity titer of 5.8 log10 FFU/mL, grown under serum-containing (SC) conditions [39], was used to inoculate CelCradle™ cultures, shake flask cultures, and for virus characterization experiments. A fourth passage virus stock with an infectivity titer of 5.1 log10 FFU/mL [32], grown under SF conditions [39], was used for virus characterization experiments.
Source from: https://www.mdpi.com/2076-393X/10/2/249/htm
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.