English
Fil-kultura taċ-ċelluli fuq skala kbira, fabbriki taċ-ċelluli huma konsumabbli użati b'mod komuni, li jintużaw prinċipalment għall-kultura ta 'ċelloli aderenti. It-tkabbir taċ-ċelluli jeħtieġ varjetà ta 'nutrijenti, allura x'inhuma?
1. Medju tal-kultura
Il-medju tal-kultura taċ-ċelluli jipprovdi liċ-ċelloli fil-fabbrika taċ-ċelluli b'nutrijenti meħtieġa għat-tkabbir, inklużi karboidrati, aċidi amminiċi, melħ inorganiku, vitamini, eċċ. Skond il-ħtiġijiet nutrittivi ta 'ċelloli differenti, hemm varjetà ta' sintetiċi midja minn fejn tagħżel, bħal EBSS, Eagle, MEM, RPMll640, DMEM, eċċ.
2. Addittivi oħra
Flimkien man-nutrijenti bażiċi pprovduti minn diversi mezzi sintetiċi, jeħtieġ li jiżdiedu komponenti oħra, bħas-serum u l-fatturi skont ċelloli differenti u skopijiet ta’ kultura differenti.
Serum jipprovdi sustanzi importanti bħall-matriċi extraċellulari , fatturi tat-tkabbir u transferrin, u serum bovin tal-fetu huwa komunement użat. Il-proporzjon tas-serum miżjud għandu jiġi determinat skond ċelluli differenti u skopijiet ta 'riċerka differenti. 10% ~ 20% serum jista 'jżomm it-tkabbir rapidu u r-rata ta' proliferazzjoni taċ-ċelloli, li tissejjaħ medju tat-tkabbir; sabiex jinżamm tkabbir bil-mod jew immortalità taċ-ċelluli, jista 'jiġi miżjud 2% ~ 5% serum, li jissejjaħ medju ta' manutenzjoni.
Glutamine hija sors importanti ta 'nitroġenu għat-tkabbir taċ-ċelluli u għandha rwol importanti fil-proċess tat-tkabbir taċ-ċelluli u l-metaboliżmu. Madankollu, minħabba li l-glutamina hija instabbli ħafna u faċilment degradata f'soluzzjoni, madwar 50% tal-glutamina tista 'tiġi dekomposta wara 7 ijiem f'4°C. Amino amide jeħtieġ li jiġi miżjud qabel l-użu.
B'mod ġenerali, diversi mezzi u serum jintużaw għall-kultura taċ-ċelluli, iżda sabiex tiġi evitata l-kontaminazzjoni taċ-ċelluli matul il-kultura, ċertu ammont ta 'antibijotiċi, bħal peniċillina, streptomycin, gentamicin, eċċ., huma miżjuda wkoll mal-mezz tal-kultura.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.