English
Flasks tal-kultura taċ-ċellulihuma prinċipalment użati għall-kultura taċ-ċelluli fuq skala żgħira u medja, allura x'tip ta 'ċelluli jistgħu jiġu kkultivati fi fliexken tal-kultura taċ-ċelluli?
Hemm ħafna tipi ta' ċelloli, li huma maqsuma f'ċelloli aderenti u ċelloli ta 'sospensjoni skond ix-xejriet tat-tkabbir tagħhom. It-tkabbir ta 'ċelluli aderenti għandu jkollu wiċċ ta' appoġġ li jista 'jitwaħħal miegħu, u ċ-ċelloli jiddependu fuq fatturi ta' twaħħil imnixxija minnhom infushom jew ipprovduti fil-mezz ta 'kultura. Sabiex jikbru u jirriproduċu fuq dan il-wiċċ. It-tkabbir taċ-ċelluli tas-sospensjoni ma jiddependix fuq il-wiċċ tas-sostenn u jikber f'sospensjoni fil-mezz tal-kultura. Il-garafini tal-kultura taċ-ċelluli huma ġeneralment magħmula minn materja prima tal-polistirene trasparenti. Speċifikazzjonijiet komuni jinkludu 25cm2, 75cm2, 175cm2, u 225cm2. Il-wiċċ tal-qiegħ tal-flask taċ-ċelluli huwa ddisinjat b'forma ta 'ark biex inaqqas l-angoli mejta ta' kuntatt meta jqiegħdu l-pipetta taċ-ċelloli. Jista 'jintuża kemm għall-kultura ta' ċelloli aderenti kif ukoll għall-kultura statika ta 'ċelloli ta' sospensjoni. It-tipi ta 'ċelluli applikabbli huma kif ġej:
Ċelloli aderenti: ċelluli Vero, ċelluli HEK 293, ċelluli CAR-T, MRC5, ċelluli CEF, makrofaġi alveolari porċini, ċelluli majeloma, ċelluli DF-1, ċelluli ST, ċelluli PK15, Marc145 ċelluli, eċċ.
Ċelloli ta 'sospensjoni: ċelluli CHO, ċelluli ta' insetti, ċelluli BHK21 u ċelluli MDCK, eċċ.
Ta 'min jinnota li jekk il- Flasks tal-kultura taċ-ċelluli jintużaw għall-kultivazzjoni ta' ċelluli aderenti, il-wiċċ tal- flixkun jeħtieġ li jiġi modifikat b'mod speċjali biex iżid l-idrofiliċità tiegħu biex jilħaq il-karatteristiċi tat-tkabbir taċ-ċelloli aderenti. Meta jintuża fil-kultura taċ-ċelluli tas-suspensjoni, mhu meħtieġ l-ebda trattament speċjali.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.