Xi jfisser li l-garafini tal-kultura taċ-ċelluli huma ħielsa mill-piroġeni?

Wed May 18 12:13:36 CST 2022

Flasks tal-kultura taċ-ċellulihuma tip ta 'konsumabbli użati komunement fil-proċess tal-kultura taċ-ċelluli. Cell tkabbir għandu rekwiżiti ogħla għall-ambjent, u l-għażla ta 'konsumabbli għandha wkoll tissodisfa r-rekwiżiti ta' mingħajr piroġenu. Imbagħad, xi jfisser ħielsa mill-piroġeni?

Hemm żewġ tipi ta 'piroġeni. Wieħed huwa l-piroġenu endoġenu, li jaġixxi fuq iċ-ċentru tar-regolazzjoni tat-temperatura tal-ġisem tal-ipotalamu u huwa proteina li tista 'tgħolli t-temperatura tal-ġisem. Wieħed huwa piroġenu eżoġenu, li jirreferi għal sustanzi barranin li jistgħu jkunu l-kawża tad-deni. Normalment jaħdem billi jinduċi piroġeni endoġeni. Dak li s-soltu nsejħu konsumabbli mingħajr piroġenu bħal fliexken tal-kultura taċ-ċelluli jirreferi għal piroġeni eżoġeni.

What does it mean that the cell culture flasks are pyrogen-free?

Piroġeni eżoġeni huma prinċipalment piroġeni batterjali, li huma metaboliti ta 'ċerti batterji, katavri batterjali u endotossini. Bacilli gram-negattivi għandhom l-abbiltà l-aktar qawwija li jikkawżaw is-sħana, segwiti minn baċilli gram-pożittivi. Cocci Gram-pożittivi huma aktar dgħajfa. Moffa, ħmira, u anke viruses jistgħu wkoll jipproduċu piroġeni. Il-piroġenu huwa ġeneralment kumpless iffurmat mill-kombinazzjoni ta 'fosfolipid polyol u proteina. Phospholipid polyol huwa ċ-ċentru attiv tal-kumpless u għandu l-effett piroġeniku l-aktar qawwi. Il-kompożizzjoni kimika tiegħu tvarja skond razez differenti. Il-piż molekulari huwa 5 × 104 sa 5 × 105, u iktar ma jkun viċin il-piż molekulari, iktar ikun qawwi l-effett termali. Fil-proċess tal-kultura taċ-ċelluli, jekk il-konsumabbli jkun fihom piroġeni, jaffettwaw direttament it-tkabbir u r-riproduzzjoni taċ-ċelloli, għalhekk dawn il-konsumabbli għandhom jgħaddu minn trattament speċjali biex ineħħu endotossini eżoġeni possibbli u jiżguraw it-tkabbir normali taċ-ċelloli.

Barra minn hekk Flasks tal-kultura taċ-ċelluli, konsumabbli bħal mikropipetti u ponot li jaqblu użati fil-kultura taċ-ċelluli jeħtieġu trattament speċjali biex jeliminaw il-piroġeni qabel ma jkunu jistgħu jintużaw.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.