English
Cell Factoryhija kultura taċ-ċelluli b'ħafna saffi konsumabbli adattata għal kultura taċ-ċelluli fuq skala kbira. Minbarra ambjent speċifiku għall-kultura taċ-ċelluli, il-meraq diġestiv huwa wkoll soluzzjoni essenzjali.
Fil-kultura in vitro taċ-ċelloli tat-tessuti u t-tixrid taċ-ċelloli tat-tessuti fil-kultura taċ-ċelluli primarji (il-blokka tat-tessuti hija ppreparata f'sospensjoni ta 'ċellula waħda) kif ukoll f'subkultura, id-diġestjoni u t-tixrid ta' ċelluli li qed jikbru aderenti għandhom jużaw soluzzjoni ta 'diġestjoni taċ-ċelluli tat-tessuti. Is-soluzzjoni tad-diġestjoni użata fit-teknoloġija tal-kultura tal-fabbrika taċ-ċelluli hija prinċipalment trypsin, EDTA, eċċ.
Il-komponenti ewlenin tal-membrana taċ-ċelluli u l-adeżjoni bejn iċ-ċelloli huma lipoproteini u polisakkaridi. Trypsin fi trypsin jista 'jikkonverti l-proteini f'peptoni, u l-lipase pankreatiku jista' jiddekomponi xaħam f'gliċerol u aċidi grassi, u b'hekk jissepara ċ-ċelloli. Iċ-ċelloli huma lisati. Il-funzjoni tas-soluzzjoni tad-diġestjoni hija prinċipalment li tidrolizza l-proteini interċellulari (bħal matriċi extraċellulari), biex tferrex it-tessut jew ċelluli aderenti f'ċelluli singoli, u biex tagħmel sospensjoni taċ-ċelluli għal aktar esperimenti.
L-enzimi diġestivi għandhom rwol importanti fil-kultura tal-fabbriki taċ-ċelluli, iżda għandu jiġi nnutat li ċelloli differenti għandhom risponsi differenti għall-enzimi tal-frixa. Meta l-valur tal-pH huwa 8.0 u t-temperatura hija 37 °C, is-soluzzjoni tal-enżima tal-frixa għandha l-aktar effett qawwi. , il-konċentrazzjoni, it-temperatura u l-ħin għandhom ikunu kkontrollati tajjeb meta jintużaw, sabiex tiġi evitata diġestjoni eċċessiva li taffettwa l-aderenza u t-tkabbir taċ-ċelloli.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.