English
Flasks tal-kultura taċ-ċellulihuma tip ta 'konsumabbli tal-kultura taċ-ċelluli użati komunement fil-kultura taċ-ċelluli aderenti. Iċ-ċelloli aderenti huma differenti miċ-ċelloli tas-sospensjoni u jeħtieġ li jitwaħħlu mal-wiċċ ta 'appoġġ biex jikbru. L-effett ta 'aderenza jaffettwa direttament it-tkabbir taċ-ċelloli, u l-effett ta' aderenza taċ-ċelloli jista 'jitjieb bil-metodi li ġejjin:
1. Diġestjoni xierqa taċ-ċelloli: Iċ-ċelloli tal-kanċer HepG-2, A549, Hela, SGC-7901 jistgħu jiġu ttrattati għal perjodu itwal ta 'żmien. Ġeneralment, il-ħin tat-trattament huwa 5-6 minuti. C2C12, COS-7, NIH-3T3 huma aktar faċli biex jaqgħu, u 2 minuti huma biżżejjed. , ċelluli P19Cl6 u 293 ma jaderixxux sewwa, sabiex ikunu jistgħu jiġu sostitwiti b'medja friska direttament wara t-tlaħliħ fil-PBS biex tferrexhom.
2. Uża fliexken tal-plastik tal-kultura taċ-ċelluli kemm jista 'jkun. Wara li fliexken bħal dawn jiġu ttrattati b'TC, il-prestazzjoni tal-adeżjoni tal-fliexken tista 'tittejjeb ħafna, u ċ-ċelloli jistgħu jitwaħħlu aħjar magħhom.
3. Resuxxita ċelloli ġodda u uża 20% serum biex tgħin liċ-ċelloli jirkupraw fl-ewwel ġimgħa.
4. Uża matriċi extraċellulari xierqa jew reaġenti ta' adeżjoni biex tgħin liċ-ċelloli jaderixxu aħjar mal-ħajt.
5. Ipprova ma tħawwadx iċ-ċelloli fi żmien 24 siegħa mill-mogħdija, sabiex ma taffettwax l-effett tal-adeżjoni taċ-ċelluli.
6. Iċ-ċelloli epiteljali fil-ġisem jikbru fuq il-membrana tal-kantina magħmula mill-kollaġen, għalhekk il-kultura fuq is-sottostrat bil-kollaġen hija ta 'benefiċċju għat-tkabbir. Għal kultura ta 'ċelluli epidermali tal-bniedem jew tal-ġurdien, ċelluli 3T3 irradjati b'raġġi γ jistgħu jintużaw bħala saff alimentatriċi, u l-pH, il-kontenut ta' Ca2+ u t-temperatura huma mnaqqsa, li huwa ta 'benefiċċju għat-tkabbir taċ-ċelluli epiteljali.
Hemm diversi modi biex ittejjeb l-effett ta 'adeżjoni taċ-ċelloli fil-Flask tal-kultura taċ-ċelluli. Barra minn hekk, kun żgur li tinfirex b'mod uniformi matul is-sottokultura biex tevita t-tkabbir taċ-ċelluli li jġorru.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.