English
2. Materjali u Metodi
2.1. Manutenzjoni ta 'Huh7.5 Cells
Huh7.5 kulturi taċ-ċelluli nżammu fi cell culture T flasks f'medju li fih is-serum (SCM): Dulbecco's Modified Eagle Medium (DMEM) (Gibco, Paisley, UK) li fih 4 mM GlutaMAX u supplimentat b' 10% serum bovin fetali (Sigma, St. Louis, MO, USA), 100 U/mL peniċillina u 100 µg/mL streptomycin (Sigma). Fi fliexken T175 (Nunc, Roskilde, id-Danimarka) u fliexken tal-kultura ta 'saff triplu (Nunc), ċelluli Huh7.5 ġew mgħoddija kull 2-3 ijiem. Il-kulturi taċ-ċelluli kollha nżammu f'37 °C u 5% CO2.
2.2. Ħażniet tal-Virus HCV
F'dan l-istudju ntużaw żewġ ħażniet ikkonfermati bis-sekwenza tal-ġenotip 5a rikombinanti SA13/JFH1core-NS5B [28], imkabbra f'kulturi ta 'ċelluli Huh7.5 monosaff f'kunjett T. Stokk tal-virus tat-tielet passaġġ b'titru ta 'infettività ta' 5.8 log10 FFU/mL, imkabbar taħt kundizzjonijiet li fihom is-serum (SC) [39], intuża biex jitlaqqam kulturi CelCradle™, ħawwad kulturi flixkun, u għal esperimenti ta 'karatterizzazzjoni tal-virus. Stokk tal-virus tar-raba’ passaġġ b’titru ta’ infettività ta’ 5.1 log10 FFU/mL [32], imkabbar taħt kundizzjonijiet SF [39], intuża għal esperimenti ta’ karatterizzazzjoni tal-virus.
Sors minn: https://www.mdpi .com/2076-393X/10/2/249/htm
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.