English
Lentivirus huwa tip ta 'vettur virali modifikat mill-virus tal-immunodefiċjenza umana (HIV), li huwa tip ta' retrovirus. F'esperimenti in vitro u esperimenti in vivo, lentivirus sar wieħed mill-forom komunement użati ta 'vettori biex jesprimu ġeni eżoġeni jew shRNA eżoġenu, u qed jintuża aktar u aktar. Fil-produzzjoni tal-massa tagħha, the fabbrika taċ-ċelluli hija konsumabbli tal-kultura taċ-ċelluli użata komunement.
Vetturi lentivirali jistgħu jintegraw b'mod effettiv ġeni eżoġeni jew shRNAs eżoġeni fil-kromożoma ospitanti, sabiex jinkiseb l-effett li jesprimu mira b'mod persistenti sekwenzi. F'termini ta 'infettività, jista' jinfetta b'mod effettiv ċelloli newronali, epatoċiti, kardjomijoċiti, ċelloli tat-tumur, ċelloli endoteljali, ċelloli staminali u tipi oħra ta 'ċelloli, sabiex jinkisbu effetti tajbin ta' terapija tal-ġeni. Għal xi ċelloli diffiċli biex jittrasfettaw, bħal ċelloli primarji, ċelloli staminali, ċelloli mhux differenzjati, eċċ., L-użu ta 'vettori lentivirali jista' jtejjeb ħafna l-effiċjenza tat-trasduzzjoni tal-ġene fil-mira jew shRNA fil-mira, u l-ġene fil-mira jew shRNA fil-mira jistgħu jiġu integrati fiċ-ċellula ospitanti. Il-probabbiltà tal-ġenoma tiżdied ħafna, u l-espressjoni fit-tul u stabbli tal-ġene fil-mira jew shRNA fil-mira tista 'tiġi realizzata b'mod aktar konvenjenti u malajr.
Il-fabbrika taċ-ċelluli hija applikata prinċipalment għall-produzzjoni estiża ta' vettori lentivirali. Jista 'jżid l-erja tal-wiċċ tal-kultura taċ-ċelluli, u b'hekk iżid ir-rendiment tal-vettur, inaqqas in-numru ta' drabi ta 'trasfezzjoni u sostituzzjoni tal-mezz ta' wara t-trasfezzjoni, u jnaqqas l-ispejjeż tax-xogħol. Hija konsumabbli ta 'kultura taċ-ċelluli effiċjenti u konvenjenti.
Minbarra li tiġi applikata għall-produzzjoni fuq skala kbira ta' vettori lentivirali, il-fabbriki taċ-ċelluli għandhom rwol importanti fil-produzzjoni fuq skala kbira ta 'terapija taċ-ċelluli, terapija tal-ġeni, terapija tal-virus onkolitiku , u vaċċini.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.