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2. Materiāli un metodes
2.1. Huh7.5 šūnu uzturēšana
Huh7.5 šūnu kultūras tika uzturētas šūnu kultūras T flasks serumu saturošā barotnē (SCM): Dulbecco's Modified Eagle Medium (DMEM) (Gibco, Paisley, UK), kas satur 4 mM GlutaMAX un papildināta ar 10% liellopu augļa seruma (Sigma, Sentluisa, MO, ASV), 100 V/mL penicilīna un 100 µg/ml streptomicīna (Sigma). T175 kolbās (Nunc, Roskilde, Dānija) un trīsslāņu kultūras kolbās (Nunc) Huh7.5 šūnas tika pasētas ik pēc 2–3 dienām. Visas šūnu kultūras tika uzturētas 37 °C temperatūrā un 5% CO2.
2.2. HCV Virus Stocks
Šajā pētījumā tika izmantoti divi genotipa 5a rekombinantā SA13/JFH1core-NS5B [28] krājumi, kas tika audzēti vienslāņu Huh7.5 šūnu kultūrās T kolbās. Trešā pasāžas vīrusa krājums ar inficētspējas titru 5,8 log10 FFU/mL, kas audzēts serumu saturošos (SC) apstākļos [39], tika izmantots CelCradle™ kultūru inokulēšanai, kolbu kratīšanas kultūrām un vīrusu raksturošanas eksperimentiem. Vīrusa raksturošanas eksperimentiem tika izmantots ceturtā pasāžas vīrusa krājums ar inficētspējas titru 5,1 log10 FFU/mL [32], kas audzēts SF apstākļos [39].
Avots no: https://www.mdpi .com/2076-393X/10/2/249/htm
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.