In cultura cellularum magnarum, ___@ consumables communiter adhibentur quae maxime ad culturam cellularum adherentium adhibentur. Cellula incrementum varietatem nutrimentorum requirit: ergo quae sunt?1. Cultura medium officinas
Cellae mediae culturae cellulas in cella officinas praebet nutrientibus, quae ad incrementum necessaria sunt, inter carbohydrates, amino acida, sales inorganici, vitamins, etc. Secundum necessitates nutritionales diversarum cellularum variae sunt syntheticae. media eligenda e, ut EBSS, Aquila, MEM, RPMll640, DMEM, etc.
2. Aliae additivae
Praeter nutrimenta fundamentalia variis instrumentis syntheticis instructis, alia membra, ut serum et factores, addere oportet secundum varias cellulas et varias culturae fines.
Serum substantias praecipuas praebet ut matrix extracellulares. , incrementa et transferrin, ac seri foetus bovinum vulgo usurpatur. Proportio seri addita debet secundum diversas cellulas et varias inquisitionis fines determinari. 10%~20% seri potest incrementum et multiplicationem celeritatis cellularum conservare, quae incrementum medium appellatur; ut tardum incrementum vel immortalitatem cellularum servet, 2%~5% seri addi potest, quod medium conservativum appellatur.
Glutamine est fons nitrogenii magni momenti pro incrementi cellae ac munere magni ponderis agit in processu incrementi cellae. et metabolismi. Sed quia glutamine valde inconstans est et in solutione facile degradatur, circa L% glutamine post 7 dies in 4°C dissolvi potest. Amino amide ante usum addi oportet.
In genere, varia media et serum pro cultura cellularum adhibentur, sed ne contagione cellae in cultura, antibioticorum aliqua copia, ut penicillinum, streptomycin, gentamicin; etc., additae sunt etiam culturae mediae.
In general, various media and serum are used for cell culture, but in order to prevent cell contamination during the culture, a certain amount of antibiotics, such as penicillin, streptomycin, gentamicin, etc., are also added to the culture medium.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.