English
Cum varias cellulas colunt, ___@ cellae quaedam culturae consumuntur quae frequentius adhibentur. Plerumque adhibentur ad culturam cellularum adherentium, et specificationes ab 25ml usque ad 225ml vagantes. Medii culturae renis cellae cricetae hoc consumabili utentur, et gradus specifici culturae eius sunt hoc modo: 1. Praeparatorium opus culturae cellae:cell culturae ampullae
1) MEM medium para; foetus bovinus serum qualis summus, 10%; duplex anticorpus 1%.
2) Condiciones culturales: Gas phase: aer, 95%; dioxide, 5%. Temperature: 37 gradus Celsius, incubator humiditatis 70%-80%.
3) Solutio geliana: 90% serum, 10% DMSO, ad usum promptum.
2. Cellulae processus:
1) Recuperatio cellularum cryopraeservarum: Celeriter excutite et tubus cryopraeservatus in quo 1 mL suspensionis cellularum in aqua balnei 37°C, adde fistulam centrifugii continentem 4-6 mL medii perfecti et misce bene. Centrifugium ad 1000RPM pro 3-5min, supernatantem abiicias, et cellulas in medio perfecto resuspendas. Suspensio cellula cellulae culturae ampullae continens 6-8 ml integrae mediae et excultae ad 37°C pernoctare tunc addita est. Cellula incrementum et densitas cellularum sub microscopio postridie observatae sunt.
2) Locus cellula: Si densitas cellae attingit 80%-90%, subculturari potest.
3) Cryopreservatio cellularum: Recepta. cellulae, commendatur seges cellularum seminum in primis 3 locis culturae ad experimenta sequentium congelascere.
Superiores sunt gradus peculiares ad colendi cellas renes in cellulis culturae ampullas. Praeterea cum cellae cryopriae conservantur, cellulas digestas in tubum centrifugium secundum processum passagii cellularum colligent, et hemocytometro utuntur ad numerandum eas ad densitatem cellularum cryopreservationem determinandam. Densitas congelatio cellularum generalium commendatarum est 1×106~1×107 cellulae viabiles/ml.
The above are the specific steps for culturing hamster kidney cells in cell culture flasks. In addition, when cells are cryopreserved, collect the digested cells into a centrifuge tube according to the process of cell passage, and use a hemocytometer to count them to determine the cryopreservation density of cells. The recommended freezing density of general cells is 1×106~1×107 viable cells/ml.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.