English
The ___@ cellula quaedam est consumabilis quae in vita investigationis et usu adhibetur. Ad cellulas adhaerentium colendas maxime usus est. In usu, modus rectae operationis requiritur ad lenis experimenti progressus. Sequentia attende cum hoc consumabili utens Quota hora est:1. Circa operculum: Noli operculum e culturae cellulae efficiendae lenticulam nimis arcte ut gas commutationem obstringere. HEPES quiddam non-ionicum est cum capacitate bona buffering in pH extensione 7.2-7.4, sed valde carus est et potest esse toxicus aliquibus cellulis in concentratione alto. HEPES quiddam adhiberi potest cum sodium carbonatis humili gradu (0.34g/L) ad incrementum pressionis osmoticae causato HEPES adiecta. Sub hac culturae conditione, operculum culturae cellulae proponendi lagena coarctari debet ne parva quantitas carbonatis in solutione culturae ne in aerem dispergatur. Plurimae culturae humores phenol rubentes continent sicut pH indicator, culturae acidicae humores sunt aurantiaco-flavi, et humores culturae alcalini sunt obscure rubentes. culturam cellularum lenticulam
2. Redigendum "incrementum cavum": Plerique utentes utentes non praeiacentibus vasculis culturae cellae efficiendis, sed in vasculis culturae cellulis ordinariae disponibilibus. Ambitus superficies mesenchymatis cellam crustulum in serum liberorum culturae ambitum non confert. murus. Pone eam ad 37°C pro 5-10 minutis ut melius coniungas substantias adhaesiones promovendas in medio culturae cum fundo culturae cellulae disposable lagena, quae facultatem adhaesionis mesenchymalis cellulis auget ac probabilitatem "incrementa foraminum minuit". ".@___@3. Cellula digestio: Pone utrem rectum et sugere culturam medium. Si plures cellulae exfoliatae in medio culturae sint, significat cellulas nunc facile delabi. Tantum adde 1ml PBS+EDTA (0,02%) et utrem culturae culturae ultro citroque excutias, donec Cellulae penitus defluant, 10ml MEM adde, bene misce, ne pipettam in 2 utres dividas, si cellulae sine incrustatione decidunt; mox adde 5ml MEM, nulla sarcina separata. Si cellulae in utre firmiter adhaerent, medium supernatantem cellam obstantiam non habet, et cellulae supra 80% obsitae sunt, medium aspirant, adde 5ml PBS+EDTA (0,02%), cito eam leviter excutite, et culturam illam Rectum Aspirate utrem, adde 1ml of 0.05% pancreatinum, digestum 37°C non plus quam 3 minuta, et cellulae omnino concoquuntur et extracta erunt. Eximito et 10ml culturae mediorum adice ad fistulam leniter et admisce, et in 2 utres divide.
Cellae valde sensitivae ad incrementum environment. Ut usitatius usu consumabilis, ___@ diligenter exerceatur et stricte praescriptas rationes sequantur ad vitandas operationes improprias quae cellam incrementum afficiunt.
ampullas culturae cellularum
Cells are very sensitive to the growth environment. As a more commonly used consumable, cell culture flasks should be operated carefully and strictly follow the prescribed procedures to avoid improper operations that affect cell growth.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.