English
Bacteria coliformia referunt ad globom bacteria gram-negativa non-bacilla quae lactosum fermentare potest, acidum et gas, aerobicum et anaerobicum facultativam producere potest. In usu adhibitis, saepe indicio ponitur num cibus a faecibus exturbandus est. Inter omnes methodos deprehendendi, usus ___@ deprehendendi communior usus est. Vestigia operationis sunt haec: 1. Sampling and dilution cellae culturae ampullae
(1) Secundum asepticam methodum pone 25g (vel 25mL) experimenti specimen in vasculo culturae cellulae continente 225mL aqua sterilis (praeset conveniens numerus globuli vitrei in lenticula) vel mortarium sterilium; et penitus excutite. Aut tere ut dilutionem uniformem 1:10. Praestat homogenizeris pro solidis exemplis ac centrifugis uti in velocitate 8000
～10000r/min pro 1 min facere 1:10 dilutionem.(2) Utere paleis sterilibus 1mL exsugere 1Ml of 1:10 dilutionem, infunde in tubulum aquae sterilis 9mL continens, excutere et miscere ad dilutionem 1:100, repone cum paleis sterilibus 1mL, premunt Operationes superiores successive fiunt 10 vicibus Vide dilutiones. .
(3) Secundum cibum hygiene requisita seu aestimationem contagionis exemplorum probati, tria dilutiones et inoculatas 3 fistulas pro singulis dilutionibus elige. Potest etiam inoculari directe cum sample.
2. Primae fermentationis probatio lactosae
Vulgo haec probatio hypothetica refertur. Propositum est exemplum in bacteria quae lactosum fermentat ad gas producendum. Specimen ut probetur, inoculatur in tubus eliquationis salis lactosae bilis, tubus fermentatus bilis lactosus duplicatus pro inoculo supra 1 mL adhibetur; una bilis lactosa tubus fermentatus salis pro 1 mL et minus quam 1 ml. Inoculate 3 tubos pro qualibet dilutione et eas pone in 36±1℃ incubatis pro 24±2h. Si omnes bilis lactosae fistulae fermentum salsae gasi non gignunt, affirmari potest pro bacteria coliformi negativa. Si gas producens est, sequitur modum procedendi infra.
3. Separatio culturae
Fabulae gignentium fermentationem striatae et inoculatae in laminas methylenae caeruleae agaro, et exculta in incubatore 36±1°C pro 18-24 horis. Tum, morphologia coloniae observata et Gram maculata, examen microscopicum et probatio recreationis fiebant.
4. Lactosum duplicatum fermentationem test
Testimonium confirmationis sic dictae, cuius propositum est probare gram-negativum non-bacillum bacteria solitarium a tubo testiculari, quod erat positivum in testi lactoso initiali fermenti, immo lactosum ad gas producendum fermentare. In medio praedicto selectivo EMB, 1 ad 2 colonias coliformes suspectas collige ad Gram maculas, et simul inoculate tubulas fermentationes lactosas, et pone eas in incubatore 36±l°C per 24±2 horas ad observandum vaporem. Fabricatio. . Ubi tubus lactosus fermentationis gasi et macula gram negativa producit, Bacillus bacteria libera pro coliformibus affirmativa referuntur; ubi lactosus fermentationis tubus gas aut gram maculam positivam non producit, pro coliformibus negativa traditur.
Haec est operandi modus utendi ___@ ad deprehendendas E. coli indices coetus. Escherichia coli magni momenti indicator est quod reflectit an cibus contaminatus sit. Methodus deprehendendi dominandi arte praecipua est ut omnis cibi inspector possideat.
Vasa culturae cellularumcell culture flasks to detect E. coli group indicators. Escherichia coli is an important indicator that reflects whether food is contaminated. Mastering the detection method is a basic skill that every food inspector should possess.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.