English
Locus celi significat processum dividendi culturam in partes parvas, re-semen in vase culturae alio, et postea in cultura. @___@ communes sunt consumabiles in processu obeundi, quare sunt gradus specifici cellae transeuntis?1. Cellulam culturae ampullas ab incubatore tolle, imbre 75% alcohol in superficie utrem cum imbre alcohol potest, eamque ad mensam ultra-mundam transfer.Cella culturae ampullae
2. Operculum aperi, medium culturae veteris sensim aspirate, congruam quantitatem adde PBS quiddam ad lavandum 1-2 temporibus cum fistula Pasteur, et quiddam PBS abiicias.
3. Utere pipettino 1-2ml pancreatinorum addere et "crucem" movere ut cellulas pancreatas plene contact.
4. Vasculum culturae cellae cum trypsin transfer ad scaenam microscopii inversam et digestionem cellam sub microscopio observa. Cum cellae rotundae fiunt et magna quantitate decidunt, concoctionem terminare parant. Superficiem utrem cum 75% alcohol sparge et eam in super Net Taiwan transfer.
5. Utere pipette (vel Pasteur pipette) aequa portione serum continentis medium ad digestionem terminandam. Pipette penitus ut cellulas totaliter defluant.
6. Mixtum liquorem in utre ad tubum centrifugium transfer, eum libra, et centrifugium circiter 5 minuta.
7. Post centrifugationem, superficies tubi centrifugii cum imbre alcoholis asperget, eam ad mensam ultramundanam transferre, operculum aperire, et supernatantem in piscinam vacuam effundere.
8. Congruum volumen addere medio seri continentis ac leniter pipettum cum pipette vel Pasteur pipettum ad suspensionem cellam faciendam.
9. Cellam suspensionem divide in 2 (vel plures) ampullas steriles culturae mundas, medium culturae debitum adde et operculum claude.
10. Vasculum culturae aliquotatum pone in stadio microscopii inversi et conditionem cellam observa. Cellarum numerus praestandus est. Nimis parvus numerus incrementum afficiet.@___@11. Nota utrem ad indicandum tempus, genus cellae, operator et alia indicia. Priusquam in incubatore ponatur, superficiem utrem corporis cum alcohol iterum sparge, mensam operantem munda, superficiem mensae ultra-mundanae cum 75% alcohole absterge, et liquida purga et purga.
The. superius est specifica processus utendi @___@ ad cellam transitum. Nota quod vestes steriles, caestus sterilium et larvarum in operatione deferant, et operas asepticas in toto processu ad evitandas cellularum contagiones.
cellam culturam laguncularum
The above is the specific process of using cell culture flasks for cell passaging. Note that you should wear sterile clothes, sterile gloves and masks during operation, and pay attention to aseptic operations throughout the process to avoid cell contamination.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.