English
Cellula cryopreservatio est ars quae cellas in ambitu humili caloris ponit ad reducere metabolismum cellularum ad longum tempus repositionis. Praeter cellas tutandas, saepe haec technologia emptio, donatio, commutatio et quaedam cellulas transportant. Ita, quomodo cellas cryopservatas transfermus ad culturam cellularum culturae cellularum?
Cellae congelatae resuscitandae sunt antequam operatio altera perfici potest. Cellarum resuscitatio ita sit ieiunium, ut cellularum viability bene conservetur. Operatio sic perfici potest:
1. Recepta cellularum siste num fasciculus exterior completus sit; exteriorem packaging aperire et in siccitate glaciei cellulas eximire;
2. Reprehendo utrum tubus cryopreservationis laedatur, an contenta liquefiant, et cito fistulam cryopraeservationis remotam in aqua balnei 37℃ liquefaciant;
3. Tubus cryopreservatio 75% alcohol disinficitur et in biologico arcae tuto collocatur;
4. 5ml medium completum ad tubulum centrifugii ad usum adde;
5 Suspensionem cellulam in tubo cryopreservationis ad medium praeparatum adde et leniter misce;
6. Tubus centrifugium pone 15mL in centrifugium et centrifugium apud 1000rpm/min (circiter 200g) pro 3-5min;
7. Adde 5ml medium completum ad 25ml vasculum culturae cellularum ad usum;
8. Cellas centrifugas tolle, sterilitatem alcoholis, et pone in armario tuto;
9. Leniter aspiras cellulam supernatantem centrifugam, 1ml medium completum ad cellulas resuspendendas adde;
10. Cellas culturae cellularum paratarum utrem nucleo resuspensum adde et suaviter misce;
11. Serva statum et densitatem cellularum inoculatarum sub microscopio, et pone culturam lenticulam in 37°C incubatore culturae.
In processu recuperationis cellae, celeri fusione efficere potest ut crystalla extracellularia brevi tempore liquescant. temporis, et damnum cellulis vitare ob tardam liquationem quae aquam in cellulas penetrare facit ad recrystallizationem intracellularem formandam. culturae ampullae cellae et aliae consumabiles usus asepticus esse debet.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.